Rat Serpin A12 Alexa Fluor® 647-conjugated Antibody

Catalog #: AF5347R Datasheet / COA / SDS

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AF5347R-100UG

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Rat Serpin A12 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity

Rat

Specificity

Detects rat Serpin A12 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross‑reactivity with recombinant human Serpin A12 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant rat Serpin A12
Leu20-Pro411
Accession # Q8R4Z1

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Serpin A12

Serpin A12 (serine proteinase inhibitor-clade A #12; also vaspin) is a 45‑50 kDa, secreted member of the alpha 1-antitrypsin (clade A)-subfamily, Serpin superfamily of protease inhibitors. It is produced by visceral and subcutaneous adipocytes, plus adipocyte-derived stem cells during obesity or diabetes. The presence of vaspin reverses insulin-resistance. Its molecular target is unknown, but it may antagonize the action of proteases that impair the action of insulin. Rat Serpin A12 is 411 amino acids (aa) in length. It contains a 20 aa signal sequence and a 391 aa mature region. A potential “active” inhibitor site lies between Gly364 and Pro389. Mature rat Serpin A12 shares 62% and 88% aa identity with human and mouse Serpin A12, respectively.

Entrez Gene IDs

145264 (Human); 68054 (Mouse); 191570 (Rat)

Alternate Names

antitrypsin), member 12; member 12; OL-64; OL-64Visceral adipose-specific serpin; serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase; Serpin A12; serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin); Vaspin; Visceral adipose tissue-derived serine protease inhibitor