Rat TSH beta Alexa Fluor® 647-conjugated Antibody

Catalog #: FAB4507R Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB4507R-100UG

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Rat TSH beta Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity

Rat

Specificity

Detects rat TSH beta in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody shows approximately 5% cross‑reactivity with recombinant human TSH beta.

Source

Monoclonal Mouse IgG2b Clone # 442506

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant rat TSH Î²
Phe21-Gly138
Accession # P04652

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: TSH beta

TSH beta is the 138 amino acid (aa), 15 kDa unique subunit of Thyroid Stimulating Hormone (TSH), which shares a common alpha subunit with other glycoprotein hormones lutropin (LH), follitropin (FSH) and chorionic gonadotropin (CG). Production of TSH by the anterior pituitary gland is stimulated by the hypothalamic peptide TRH, and inhibited by feedback from the thyroid hormones triiodothyronine (T3) and thyroxine (T4). Mature rat TSH beta shares 92% and 90% aa sequence identity with mouse and human TSH beta, respectively.

Long Name

Thyroid-stimulating Hormone Subunit beta

Entrez Gene IDs

7252 (Human); 22094 (Mouse); 25653 (Rat)

Alternate Names

thyroid stimulating hormone, beta; thyroid-stimulating hormone subunit beta; thyrotropin beta chain; thyrotropin beta subunit; Thyrotropin beta; thyrotropin subunit beta; TSH beta; TSHB; TSH-B; TSH-BETA