Recombinant Cynomolgus Monkey Fc gamma RIIB Protein, CF

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Recombinant Cynomolgus Monkey Fc gamma RIIB Protein, CF Data
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Recombinant Cynomolgus Monkey Fc gamma RIIB Protein, CF Summary

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey Fc gamma  RIIB/CD32b is immobilized on a His Tag Antibody coated plate, it binds Biotinylated Human IgG. The concentration of Biotinylated Human IgG that produces 50% of the optimal binding response is approximately 0.3-1.5 μg/mL.
Human embryonic kidney cell, HEK293-derived cynomolgus monkey Fc gamma RIIB/CD32b protein
Ala46-Pro217, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Predicted Molecular Mass
20 kDa
21-35 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity View Larger

When Recombinant Cynomolgus Monkey Fc gamma RIIB/CD32b (Catalog # 9246-CD) is immobilized on a His Tag Antibody coated plate, Biotinylated Human IgG binds with an ED50of 0.3-1.5 μg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Background: Fc gamma RIIB/CD32b

Receptors for the Fc region of IgG (Fc gamma  Rs) are members of the Ig superfamily that function in the activation or inhibition of immune responses such as degranulation, phagocytosis, ADCC (antibody-dependent cellular toxicity), cytokine release, and B cell proliferation (1). The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma  RI/CD64, Fc gamma  RII/CD32, and Fc gamma  RIII/CD16. CD32 proteins are encoded by three genes in human (Fc gamma RII A, B, and C), two in cynomolgus (Fc gamma RII A and B), and one in mouse (Fc gamma RIIB). Fc gamma RII A and C contain activating cytoplasmic ITAM sequences, while Fc gamma RIIB contains an inhibitory cytoplasmic ITIM sequence. The CD64 proteins are high affinity receptors (~10-8-10-9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10-6-10-7 M) and only recognize IgG aggregates surrounding multivalent antigens (1). The approximately 40 kDa mature cynomolgus Fc gamma RIIB consists of a 175 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 23 aa transmembrane segment, and a 51 aa cytoplasmic domain. Within the ECD, cynomolgus Fc gamma RIIB shares 90%, 64%, and 61% aa sequence identity with human, mouse, and rat Fc gamma RIIB, respectively. It binds to both cynomolgus and human IgG subclasses 1-4 (2). Fc gamma RIIB is expressed on B cells, monocytes, dendritic cells, neutrophils, mast cells, and basophils (1). Ligation of Fc gamma RIIB triggers signaling that suppresses B cell expansion, plasma cell differentiation, production of autoimmune rheumatoid factors, and down-regulation of TLR4 with reduced LPS responsiveness (3-6).

  1. Nimmerjahn, F. and J.V. Ravetch (2008) Nat. Rev. Immunol. 8:34.
  2. Warncke, M. et al. (2012) J. Immunol. 188:4405.
  3. Takai, T. et al. (1996) Nature 379:346.
  4. Karnell, J.L. et al. (2014) J. Immunol. 192:1480.
  5. Moll, T. et al. (2004) J. Immunol. 173:4724.
  6. Zhang, Y. et al. (2009) J. Immunol. 182:554.
Long Name
Fc gamma Receptor II
Entrez Gene IDs
2213 (Human); 14130 (Mouse); 289211 (Rat); 102139530 (Cynomolgus Monkey)
Alternate Names
CD32 antigen; CD32b; CD32Fc fragment of IgG, low affinity II, receptor for (CD32); CDw32; Fc fragment of IgG, low affinity IIb, receptor (CD32); Fc fragment of IgG, low affinity IIb, receptor for (CD32); Fc gamma RIIB; FCG2; Fc-gamma RII-b; fc-gamma-RIIb; FCGR2; FCGR2B; FcgRIIB; FCRIIB; fcRII-b; IGFR2; IgG Fc receptor II-b; low affinity immunoglobulin gamma Fc region receptor II-b


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