Recombinant Human Angiopoietin-like 4 Protein, CF

R&D Systems | Catalog # 4487-AN

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Key Product Details

  • R&D Systems NS0-derived Recombinant Human Angiopoietin-like 4 Protein (4487-AN)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

Q9BY76

Structure / Form

Oligomer

Applications

Inhibition Activity
View all Angiopoietin-like Protein 4/ANGPTL4 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Angiopoietin-like Protein 4/ANGPTL4 protein
Gly26-Ser406 (Lys163Ala & Arg164Ala), with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly26

Predicted Molecular Mass

43.6 kDa (monomer)

SDS-PAGE

50-55 kDa, reducing conditions

Activity

Measured by its ability to inhibit lipoprotein lipase activity. Yoshida, K. et al. (2002) J. Lipid Res. 43:1770.
The IC50 value under conditions in which Recombinant Human Lipoprotein Lipase/LPL (Catalog # 9888-LL)  and p-nitrophenyl butyrate are present in 0.1 M sodium phosphate, 0.15 M NaCl, 0.5% Triton® X-100, pH 7.2, is approximately 0.05-2.0 µg/mL.

Reviewed Applications

Read 3 reviews rated 4.7 using 4487-AN in the following applications:

Formulation, Preparation, and Storage

4487-AN
Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, NaCl and CHAPS.
Reconstitution

Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Angiopoietin-like Protein 4/ANGPTL4

Angiopoietin-like 4 (ANGPTL4), also known as FIAF, FARP, and PGAR, is a 55 kDa glycoprotein secreted by the liver and fat tissue. It is structurally related to the angoipoietins and contains an N-terminal coiled coil domain and a C-terminal fibrinogen-like domain which can be proteolytically separated in vivo (1). Mature human ANGPTL4 shares 26%-30% amino acid (aa) sequence identity with ANGPTL1, 2, 3, 5, 6, and 7. It shares approximately 75% aa sequence identity with mouse and rat ANGPT-L4. The coiled coil domain, which is not glycosylated, mediates the formation of variable sized disulfide-linked oligomers (2). This domain directly inhibits lipoprotein lipase, resulting in increased circulating triglyceride levels (3, 4). In humans, the N-terminal fragment and full length ANGPTL4 physically associate with HDL (4). In mouse, however, full length ANGPTL4 associates with HDL, while the N-terminal fragment associates with LDL (4). Circulating ANGPTL4 is decreased in type II diabetics with a subsequent loss of its normal plasma glucose lowering activity (5). Its expression in adipose tissue is induced by fasting and suppressed by feeding (6). In hypoxic areas, ANGPTL4 is induced in both vascular endothelial cells and tumor cells (7, 8). The N-terminal fragment can function as an angiogenesis inhibitor (7, 8). In contrast, the C-terminal fragment modulates cell adhesion through interactions with heparan sulfate proteoglycans, Integrins  beta 1 and beta 5, Vitronectin, and Fibronectin, thereby promoting keratinocyte migration and wound healing (7, 9, 10). ANGPTL4 additionally enhances the survival of hematopoietic and mesenchymal stem cells (11, 12). The expression of an undersialylated form of ANGPTL4 in renal podocytes contributes to proteinuria and nephrotic syndrome (13).

References

  1. Zhu, P. et al. (2012) Biosci. Rep. 32:211.
  2. Ge, H. et al. (2004) J. Biol. Chem. 279:2038.
  3. Sukonina, V. et al. (2006) Proc. Natl. Acad. Sci. USA 103:17450.
  4. Mandard, S. et al. (2006) J. Biol. Chem. 281:934.
  5. Xu, A. et al. (2005) Proc. Natl. Acad. Sci. USA 102:6086.
  6. Kersten, S. et al. (2000) J. Biol. Chem. 275:28488.
  7. Cazes, A. et al. (2006) Circ. Res. 99:1207.
  8. Le Jan, S. et al. (2003) Am. J. Pathol. 162:1521.
  9. Goh, Y.Y. et al. (2010) Am. J. Pathol. 177:2791.
  10. Goh, Y.Y. et al. (2010) J. Biol. Chem. 285:32999.
  11. Blank, U. et al. (2012) Eur. J. Haematol. 89:198.
  12. Hou, M. et al. (2014) PLoS ONE 9:e85808.
  13. Clement, L.C. et al. (2011) Nat. Med. 17:117.

Alternate Names

Angiopoietin like Protein 4, ANGPTL4, FIAF, HFARP, PGAR

Entrez Gene IDs

51129 (Human); 57875 (Mouse); 362850 (Rat)

Gene Symbol

ANGPTL4

UniProt

Q9BY76

Additional Angiopoietin-like Protein 4/ANGPTL4 Products

Product Documents for Recombinant Human Angiopoietin-like 4 Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human Angiopoietin-like 4 Protein, CF

For research use only

Citations for Recombinant Human Angiopoietin-like 4 Protein, CF

Customer Reviews for Recombinant Human Angiopoietin-like 4 Protein, CF (3)

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  • Name: Anonymous
    Application: Immunoassay Standard
    Verified Customer | Posted 11/30/2018
  • Recombinant Human Angiopoietin-like 4 Protein, CF
    Name: Ophelia Pedersen
    Application: Enzymatic activity in vitro
    Verified Customer | Posted 01/24/2017
    Recombinant Human Angiopoietin-like 4 Protein, CF 4487-AN
  • Recombinant Human Angiopoietin-like 4 Protein, CF
    Name: Allison McQueen
    Application: SDS-PAGE Control
    Verified Customer | Posted 01/14/2016

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