Recombinant Human BTN3A2 Biotinylated Protein, CF

Name: Recombinant Human BTN3A2 Biotinylated Protein, CF
Brand: R&D Systems
SKU: BT9514
Price: 469 USD

Catalog #: BT9514 Datasheet / COA / SDS

Discontinued Product

BT9514 has been discontinued.

View all BTN3A2 products.

Recombinant Human BTN3A2 Biotinylated Protein Bioactivity

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All BTN3A2 Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human BTN3A2 Biotinylated Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to enhance anti-CD3-induced IFN-gamma secretion of mouse CD3⁺ T cells. The ED₅₀ for this effect is 2-12 μg/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human BTN3A2 protein
Gln30-Trp248, with a C-terminal 6-His tag, biotinylated via sugars

Accession #

P78410-1

N-terminal Sequence
Analysis

Gln30

Predicted Molecular Mass

24 kDa (before labeling)

SDS-PAGE

26-33 kDa and 56-65 kDa, reducing conditions

Product Datasheets

BT9514

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

BT9514

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	12 months from date of receipt, ≤ -20 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Recombinant Biotinylated Human BTN3A2 (Catalog # BT9514) enhances anti-CD3 antibody induced IFN-gamma secretion by muse CD3⁺T cells. The ED₅₀ for this effect is 2-12 μg/mL.

Reconstitution Calculator

Background: BTN3A2

BTN3A2 (Butyrophilin subfamily 3 member A2; also BTF3 and BT3.2) is a 36 kDa (predicted) glycoprotein, member of the BTN family, Ig Superfamily of molecules. It is postulated to be expressed on immune-related cells, as it has a structural similarity to MHC and CD80/CD86 molecules. Mature human BTN3A2 is a 305 amino acid (aa) type I transmembrane protein. It contains a 219 aa extracellular region with one V-type Ig-like domain and a 65 aa cytoplasmic tail. The cytoplasmic region undergoes phosphorylation on two serines. There are three potential splice forms. A rodent counterpart to BTN3A2 has not been reported. BTN3A2 mRNA over‑expression was associated with a good prognosis in relation to disease-free and overall survival in a cohort of 55 epithelial ovarian cancer (EOC) patients (1). Another study in a larger cohort of 199 high-grade EOC patients further confirmed that the protein expression of BTN3A2 in ovarian cancer tissues is positively correlated with the intraepithelial infiltration of CD4⁺ and CD8⁺ T cells (2), suggesting that BTN3A2 was a co-stimulatory molecule to modulate the infiltration of immune cells and thus the anti-cancer immunity. In consistent with previous publications, our in-house studies on BTN3A2 showed that BTN3A2 co-stimulated anti‑CD3 induced IFN-gamma secretion on CD3⁺ cells.

References