Cathepsin V (also known as Cathepsin L2 and U) is a cysteine protease of the papain family (1). It is expressed as a proenzyme in lysosomes, which can be autocatalytically converted to the mature form at pH 4.0 (2). Cathepsin V shows high amino acid identity with Cathepsin L (77% for the proenzymes and 80% for the mature enzymes) (3). However, Cathepsin V is specifically expressed in the thymus, testis, and corneal epithelium whereas Cathepsin L is expressed throughout the body.
Recombinant Human Cathepsin V Protein, CF
R&D Systems | Catalog # 1080-CY
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Key Product Details
- R&D Systems NS0-derived Recombinant Human Cathepsin V Protein (1080-CY)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
NS0
Accession Number
Structure / Form
Pro form
Applications
Enzyme Activity
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Product Specifications
Source
Mouse myeloma cell line, NS0-derived human Cathepsin V protein
Val18-Val334, with a C-terminal 10-His tag
Val18-Val334, with a C-terminal 10-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Val18
Predicted Molecular Mass
37 kDa
SDS-PAGE
40 kDa, reducing conditions
Activity
Measured by its ability to cleave the fluorogenic peptide substrate Z-LR-AMC (Catalog # ES008).
The specific activity is >400 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation, and Storage
1080-CY
| Formulation | Supplied as a 0.2 μm filtered solution in Sodium Acetate and NaCl. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Cathepsin V
References
- Turk, V. et al. (2001) EMBO J. 20:4629.
- Bromme, D. et al. (1999) Biochemistry 38:2377.
- Somoza, J.R. et al. (2000) Biochemistry 39:12543.
Alternate Names
Cathepsin L2, Cathepsin U, CTSL2
Entrez Gene IDs
1515 (Human)
Gene Symbol
CTSV
UniProt
Additional Cathepsin V Products
Product Documents for Recombinant Human Cathepsin V Protein, CF
Product Specific Notices for Recombinant Human Cathepsin V Protein, CF
For research use only
Related Research Areas
Citations for Recombinant Human Cathepsin V Protein, CF
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Protocols
View specific protocols for Recombinant Human Cathepsin V Protein, CF (1080-CY):
Materials
- Assay Buffer: 25 mM Sodium Acetate, 0.1 M NaCl, 5 mM DTT, pH 5.5
- Recombinant Human Cathepsin V (rhCathepsin V) (Catalog # 1080-CY)
- Substrate: Z-Leu-Arg-AMC ((Catalog # ES008), 2 mM stock in DMSO
- F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
- Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent
- Dilute rhCathepsin V to 1 µg/mL in Assay Buffer.
- Dilute Substrate to 20 µM in Assay Buffer.
- Load into a black well plate 50 µL of 1 µg/mL rhCathepsin V and start the reaction by adding 50 µL of 20 µM Substrate.
- Read at excitation and emission wavelengths of 380 nm and 460 nm (top read), respectively, in kinetic mode for 5 minutes.
- Calculate specific activity:
Specific Activity (pmol/min/µg) =
Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU) amount of enzyme (µg) *Adjusted for Substrate Blank
**Derived using calibration standard 7-Amino, 4-Methyl Coumarin (AMC) (Sigma, Catalog # A-9891).
Per Well:
- rhCathepsin V: 0.050 μg
- Substrate: 10 µM