Recombinant Human Chondroadherin His-tag Protein, CF

R&D Systems | Catalog # 8218-CH

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Key Product Details

  • R&D Systems CHO-derived Recombinant Human Chondroadherin His-tag Protein (8218-CH)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

O15335

Applications

Bioactivity
View all Chondroadherin Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Chondroadherin protein
Ala22-Thr350, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala22 & Cys23

Predicted Molecular Mass

38 kDa

SDS-PAGE

33-38 kDa, reducing conditions

Activity

Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells.
The ED50 for this effect is 0.4-2.4 μg/mL.

Scientific Data Images for Recombinant Human Chondroadherin His-tag Protein, CF

Recombinant Human Chondroadherin His-tag Protein Bioactivity

Recombinant Human Chondroadherin His-tag Protein Bioactivity

Recombinant Human Chondroadherin (Catalog # 8218-CH) induces adhesion of ATDC5 mouse chondrogenic cells. The ED50 for this effect is 0.4-2.4 μg/mL.
Recombinant Human Chondroadherin His-tag Protein SDS-PAGE

Recombinant Human Chondroadherin His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Human Chondroadherin was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 33-38 kDa.

Formulation, Preparation, and Storage

8218-CH
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Chondroadherin

Chondroadherin (CHAD), also known as SLRR4A, is a 38-kDa secreted protein belonging to the small leucine-rich proteoglycans (SLRPs) that help regulate the assembly and function of the ECM (1). CHAD is highly expressed in cartilaginous tissues, with lower expression levels found in bone, tendon, and eye (2-4). Mature human CHAD consists of a 337 amino acid (aa) protein that contains eleven leucine-rich repeats (LRRs) including nine tandem leucine-rich repeats as well as N-terminal and C-terminal leucine-rich domains flanked by cysteine-rich regions (5). CHAD interacts with collagen II and mediates signaling between chondrocytes and the ECM by binding to the alpha 2 beta 1 integrin, heparan sulphate, and to cell surface proteoglycans like syndecans (5-9). In addition, CHAD interacts with both N- and C-terminal globular domains of type VI collagen (10). Human CHAD shares 95% and 94% aa sequence identity with mouse and rat CHAD, respectively.

References

  1. Neame, P.J. et al. (1994) J. Biol. Chem. 269:21547.
  2. Larson, T. et al. (1991) J. Biol. Chem. 266:20428.
  3. Mizuno, M. et al. (1996) Calcif. Tissue Int. 59:163.
  4. Johnson, J.M. et al. (2006) Mol. Vis. 12:1057.
  5. Månsson, B. et al. (2001) J. Biol. Chem. 276:32883.
  6. Paracuellos, P. et al. (2017) Matrix Biol. 63:106.
  7. Batista, M. et al. (2014) Matrix Biol. 38:84.
  8. Camper, L. et al. (1997) J. Cell Biol. 138:1159.
  9. Haglund, L. et al. (2013) J. Biol. Chem. 288:995.
  10. Wiberg, C. et al. (2002) J. Biol. Chem. 277:49120.

Alternate Names

CHAD, SLRR4A

Entrez Gene IDs

1101 (Human); 12643 (Mouse); 29195 (Rat)

Gene Symbol

CHAD

UniProt

O15335

Additional Chondroadherin Products

Product Documents for Recombinant Human Chondroadherin His-tag Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human Chondroadherin His-tag Protein, CF

For research use only

Citations for Recombinant Human Chondroadherin His-tag Protein, CF

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