Recombinant Human Decorin Protein, CF

R&D Systems | Catalog # 11808-DE

CHO expressed
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Key Product Details

Source

CHO

Accession Number

P07585.1

Applications

Bioactivity
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Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Decorin protein
Asp31-Lys359

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asp31

Predicted Molecular Mass

36 kDa

SDS-PAGE

38-47 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
Recombinant Human Decorin binds to collagen with an ED50 of <100 ng/mL.

Scientific Data Images for Recombinant Human Decorin Protein, CF

Recombinant Human Decorin Protein Binding Activity.

Measured by its binding ability in a functional ELISA. Recombinant Human Decorin Protein (Catalog # 11808-DE) binds to collagen with an ED50 of <100 ng/mL.

Recombinant Human Decorin Protein SDS-PAGE.

2 μg/lane of Recombinant Human Decorin Protein (Catalog # 11808-DE) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands 38-47 kDa, under reducing conditions.

Formulation, Preparation, and Storage

11808-DE
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 100 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Decorin

Decorin, also known as PG40 and PGS2, is a secreted chondroitin/dermatan sulfate proteoglycan in the family of small leucine‑rich proteoglycans (SLRPs). SLRP family members are characterized by N‑terminal and C‑terminal cysteine‑rich regions which flank the central region containing 10 ‑ 12 tandem leucine‑rich repeats (LRR) (1). The human Decorin cDNA encodes a 359 amino acid (aa) precursor that includes a 16 aa signal sequence and a 14 aa propeptide (2). Mature human Decorin contains twelve tandem LRR and shares 80% and 78% aa sequence identity with mouse and rat Decorin, respectively. Alternate splicing of human Decorin generates five isoforms with variable length deletions. Decorin is an N‑glycosylated protein that also carries a variably‑sized hybrid chondroitin/dermatan sulfate chain at Ser34 (3, 4). Naturally occurring Decorin proteoglycan has a molecular mass of approximately 100 kDa, and the deglycosylated Decorin core protein has a mass of approximately 40 kDa (5). Decorin regulates assembly of the extracellular collagen matrix and the bioactivity of the matrix associated growth factors FGF‑2, GDF‑8/Myostatin, TGF‑ beta, and WISP‑1 (4, 6 ‑ 9). It also binds and activates EGF R, ErbB4, and IGF‑I R (10 ‑ 12). In vivo, Decorin promotes myoblast differentiation, supports angiogenesis, and inhibits tumor progression (13 ‑ 16). Decorin is cleared from the extracellular space by LRP‑mediated endocytosis (17).

References

  1. Schaefer, L. and R.V. Iozzo (2008) J. Biol. Chem. 283:21305.
  2. Krusius, T. and E. Ruoslahti (1986) Proc. Natl. Acad. Sci. 83:7683.
  3. Scholzen, T. et al. (1994) J. Biol. Chem. 269:28270.
  4. Zamfir, A. et al. (2003) Glycobiology 13:733.
  5. Roughley, P.J. and R.J. White (1989) Biochem. J. 262:823.
  6. Ferdous, Z. et al. (2007) J. Biol. Chem. 282:35887.
  7. Miura, T. et al. (2006) Biochem. Biophys. Res. Commun. 340:675.
  8. Cabello-Verrugio, C. and E. Brandan (2007) J. Biol. Chem. 282:18842.
  9. Desnoyers, L. et al. (2001) J. Biol. Chem. 276:47599.
  10. Zhu, J.-X. et al. (2005) J. Biol. Chem. 280:32468.
  11. Santra, M. et al. (2000) J. Biol. Chem. 275:35153.
  12. Schonherr, E. et al. (2005) J. Biol. Chem. 280:15767.
  13. Olguin, H.C. et al. (2003) Dev. Biol. 259:209.
  14. Schonherr, E. et al. (2004) J. Vasc. Res. 41:499.
  15. Seidler, D.G. et al. (2006) J. Biol. Chem. 281:26408.
  16. Reed, C.C. et al. (2005) Oncogene 24:1104.
  17. Brandan, E. et al. (2006) J. Biol. Chem. 281:31562.

    Alternate Names

    DCN, DSPG2, PG-II, PGS2, SLRR1B

    Entrez Gene IDs

    1634 (Human); 13179 (Mouse)

    Gene Symbol

    DCN

    UniProt

    P07585.1

    Additional Decorin Products

    Product Documents for Recombinant Human Decorin Protein, CF

    Certificate of Analysis

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    Product Specific Notices for Recombinant Human Decorin Protein, CF

    For research use only

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