Recombinant Human EphA3 Fc Chimera Protein, CF

R&D Systems | Catalog # 6444-A3

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Key Product Details

  • R&D Systems NS0-derived Recombinant Human EphA3 Fc Chimera Protein (6444-A3)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

NP_005224

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all EphA3 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human EphA3 protein
Human EphA3
(Met1 - Gln541)
Accession # NP_005224
 IEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu21

Predicted Molecular Mass

85.4 kDa (monomer)

SDS-PAGE

100-110 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.

When Recombinant Human (rh) EphA3 Fc Chimera is coated at 2 μg/mL (100 μL/well), the concentration of biotinylayed rhEphrin A5 Fc Chimera that produces 50% of the optimal binding response is found to be approximately 5 ‑ 25 ng/mL.

Formulation, Preparation, and Storage

6444-A3
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: EphA3

EphA3, also known as Cek4, Mek4, Hek, Tyro4, and Hek4, is a 135 kDa glycosylated member of the transmembrane Eph receptor tyrosine kinase family. The A and B classes of Eph proteins are distinguished by Ephrin ligand binding preference but have a common structural organization. EphA3 preferentially binds to Ephrin-A5. Eph-Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression (1, 2). The 521 amino acid (aa) extracellular domain (ECD) of human EphA3 contains an N-terminal Ephrin binding region, a cysteine-rich region, and two fibronectin type II domains. The 418 aa cytoplasmic domain contains the tyrosine kinase domain and a sterile alpha motif (SAM) (3, 4). Within the ECD, human EphA3 shares 96% aa sequence identity with mouse and rat EphA3. Alternate splicing generates a secreted isoform that consists of nearly the entire ECD. EphA3 is expressed in the developing forebrain, retinal axons, some spinal cord motor neurons, and the heart where it plays an important role in axonal repulsion and organ morphogenesis (5 - 8). It is upregulated on some hematopoietic and solid tumor cells and on astrocytes surrounding injured nervous tissue (3, 5, 9 - 11). EphA3 ligation inhibits cellular adhesion to fibronectin as well as cellular migration (9, 10). Transmembrane EphA3 associates in cis with ADAM10 which then promotes the cleavage in trans of Ephrin-A5 (12). It also associates in cis with Ephrin-A5 on retinal axons, thereby preventing the activation of EphA3 by Ephrin-A (13). Multiple tyrosine residues within the cytoplasmic region of EphA3 become phosphorylated during ligand-induced signaling (14, 15).

References

  1. Pasquale, E.B. (2005) Nat. Rev. Mol. Cell Biol. 6:462.
  2. Merlos-Suarez, A. and E. Batlle (2008) Curr. Opin. Cell Biol. 20:194.
  3. Wicks, I.P. et al. (1992) Proc. Natl. Acad. Sci. 89:1611.
  4. Lackmann, M. et al. (1998) J. Biol. Chem. 273:20228.
  5. Chiari, R. et al. (2000) Cancer Res. 60:4855.
  6. Kudo, C. et al. (2005) J. Comp. Neurol. 487:255.
  7. Kilpatrick, T.J. et al. (1996) Mol. Cell. Neurosci. 7:62.
  8. Stephen, L.J. et al. (2007) Dev. Biol. 302:66.
  9. Smith, L.M. et al. (2004) Exp. Cell Res. 292:295.
  10. Clifford, N. et al. (2008) J. Cell. Biochem. 105:1250.
  11. Irizarry-Ramirez, M. et al. (2005) J. Neurotrauma 22:929.
  12. Janes, P.W. et al. (2005) Cell 123:291.
  13. Carvalho, R.F. et al. (2006) Nat. Neurosci. 9:322.
  14. Shi, G. et al. (2010) Cell Res. June epub.
  15. Hu, T. et al. (2009) Biochemistry 48:6369.

Long Name

Eph Receptor A3

Alternate Names

Cek4, Hek4, Tyro4

Entrez Gene IDs

2042 (Human); 13837 (Mouse)

Gene Symbol

EPHA3

UniProt

NP_005224

Additional EphA3 Products

Product Documents for Recombinant Human EphA3 Fc Chimera Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human EphA3 Fc Chimera Protein, CF

For research use only

Citations for Recombinant Human EphA3 Fc Chimera Protein, CF

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