Recombinant Human ErbB2/Her2 His-tag Protein, CF
Recombinant Human ErbB2/Her2 His-tag Protein, CF Summary
Ser22-Thr652, with a C-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 500 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
ErbB2, also called Neu and Her2 (human epidermal growth factor receptor 2), is a type I transmembrane glycoprotein member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factors. It is widely expressed in epithelial cells and has also been found to be over-expressed in a large number of breast carcinomas. Among the ErbB family members, ErbB2 is unique in that it has no identified ligands. Rather, ErbB2 heterodimerizes with the other members of the ErbB family (ErbB1 (EGFR), ErbB3, ErbB4) to form higher affinity signaling complexes. Human ErbB2 consists of 1255 amino acids (aa) including a 22 aa signal sequence, a 630 aa extracellular domain (ECD), a 23 aa transmembrane region, and a 580 aa cytoplasmic domain. Within the ECD, human ErbB2 shares and 86% aa sequence identity with mouse and rat ErbB2. Phosphoinositide 3-kinase has been shown to play a role in ErbB2 signal transduction. The cytoplasmic domain of ErbB2 has been shown to associate with beta-catenin and plakoglobin. ErbB2 appears to play roles in development, cancer, communication at the neuromuscular junction and regulation of cell growth and differentiation (1-10).
- Coussens, L. et al. (1985) Science 230:1132.
- Yamamoto, T. et al. (1986) Nature 319:230.
- Kanai, Y. et al. (1995) Biochem. Biophys. Res. Commun. 208:1067.
- Codony-Servat, J. et al. (1999) Cancer Res. 59:1196.
- Carraway, K.L. 3rd et al. (1994) J. Biol. Chem. 269:14303.
- Emkey, R. and C.R. Kahn (1997) J. Biol. Chem. 272:31172.
- Schaefer, G. et al. (1999) J. Biol. Chem. 274:859.
- Schlessinger, J. (2000) Cell 103:211.
- Hellyer, N.J. et al. (2001) J. Biol. Chem. 276:42153.
- Daly, R.J. (1999) Growth Factors 16:255.
Citations for Recombinant Human ErbB2/Her2 His-tag Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 3
Filter your results:
Inducible expression of interleukin-12 augments the efficacy of affinity-tuned chimeric antigen receptors in murine solid tumor models
Authors: Y Yang, H Yang, Y Alcaina, J Puc, A Birt, Y Vedvyas, M Gallagher, S Alla, MC Riascos, JE McCloskey, K Du, J Gonzalez-V, IM Min, E de Stanchi, M Britz, E von Hofe, MM Jin
Nature Communications, 2023-04-12;14(1):2068.
Sample Types: Whole Cells
Spatial-Division Multiplexing Approach for Simultaneous Detection of Fiber-Optic Ball Resonator Sensors: Applications for Refractometers and Biosensors
Authors: M Shaimerden, T Ayupova, A Bekmurzaye, M Sypabekova, Z Ashikbayev, D Tosi
Sample Types: Serum
An antibody Fc engineered for conditional antibody-dependent cellular cytotoxicity at the low tumor microenvironment pH using mammalian cell display
Authors: Y Liu, AG Lee, AW Nguyen, JA Maynard
The Journal of Biological Chemistry, 2022-03-03;0(0):101798.
Species: Human, Transgenic Mouse
Sample Types: Serum, Whole Cells
Applications: ELISA Capture, Flow Cytometry
No product specific FAQs exist for this product, however you mayView all Proteins and Enzyme FAQs
Reviews for Recombinant Human ErbB2/Her2 His-tag Protein, CF
There are currently no reviews for this product. Be the first to review Recombinant Human ErbB2/Her2 His-tag Protein, CF and earn rewards!
Have you used Recombinant Human ErbB2/Her2 His-tag Protein, CF?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image