Recombinant Human His10-USP47 Protein, CF

Name: Recombinant Human His10-USP47 Protein, CF
Brand: R&D Systems
SKU: E-626
Rating: 5 (1 reviews)

Catalog #: E-626 1 Review Datasheet / COA / SDS

Discontinued Product

E-626 has been discontinued.

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human His10-USP47 Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Activity

Recombinant Human USP47 is a Ubiquitin deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial concentration of 10-100 nM USP47 when using Ubiquitin-AMC (U-550) or Ubiquitin-Rhodamine (U-555) as a substrate.

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human USP47 protein
Contains an N-terminal 10-His tag

Predicted Molecular Mass

160 kDa

Product Datasheets

E-626

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E-626

Formulation	X mg/ml (X μM) in 50 mM HEPES pH 7.5, 150 mM NaCl, 1 mM TCEP
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 24 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.

Reconstitution Calculator

Background: USP47

Ubiquitin carboxyl-terminal hydrolase 47 (USP47) is a C19 type peptidase with a predicted molecular weight of 157 kDa. The human protein shares 95% and 96% amino acid sequence identity with its mouse and rat orthologs, respectively. USP47 has been reported to play roles in inflammasome activation and colon cancer progression. This recombinant protein contains an n-terminal 10-His tag.

References

Palazon-Riquelme, P. et al. (2018) EMBO Rep. doi: 10.15252/embr.201744766
Yu, L. et al. (2019) Cancer Letters doi: 10.1016/j.canlet.2019.01.039

Long Name

Ubiquitin Specific Protease 47

Entrez Gene IDs

55031 (Human); 74996 (Mouse)

Alternate Names

Deubiquitinating enzyme 47; DKFZp686C13257; EC 3.4.19.12; FLJ20727; Trf (TATA binding protein-related factor)-proximal homolog (Drosophila); Trf (TATA binding protein-related factor)-proximal homolog; TRFP; ubiquitin carboxyl-terminal hydrolase 47; ubiquitin specific peptidase 47; ubiquitin specific protease 47; ubiquitin thioesterase 47; Ubiquitin thiolesterase 47; Ubiquitin-specific-processing protease 47; USP47