Recombinant Human His6-Ataxin-3 Protein, CF
Recombinant Human His6-Ataxin-3 Protein, CF Summary
- R&D Systems E. coli-derived Recombinant Human His6-Ataxin-3 Protein (E-341)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Product Specifications
Contains an N-terminal Ser-Tyr-Tyr and 6-His tag
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
E-341
| Formulation | X mg/ml (X μM) in 50 mM HEPES pH 7.5, 150 mM NaCl, 10% Glycerol (v/v), 1mM TCEP |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: Ataxin-3
Ataxin-3, also known as Machado-Joseph Disease (MJD) Protein 1 and Spinocerebellar Ataxia Type 3, is a 364 amino acid (aa), ubiquitously expressed cytoplasmic and nuclear protein with a predicted molecular weight of 42 kDa. Ataxin-3 functions as a deubiquitinating enzyme. Human Ataxin-3 shares 87% and 86% aa sequence identity with mouse and rat Ataxin-3, respectively. Full-length Ataxin-3 contains an N-terminal Josephin domain, two Ubiquitin interacting motifs, and a variable C-terminus consisting of a polyglutamine stretch and tail (1,2). As a deubiquitinating enzyme, Ataxin-3 plays a critical role in affecting the ubiquitination status of proteins for quality control and other cellular pathways (3). In turn, the ubiquitination of Ataxin-3 is was shown to enhance its capacity to cleave Ubiquitin chains (4). By opposing the actions of the Ubiquitin-conjugating (E2) enzyme UBE2W, Ataxin-3 is believed to control the activity of the Ubiquitin ligase (E3) C-terminus of Hsp70 Interacting Protein (CHIP) (5). CHIP binds to protein chaperones and represents an important molecular link between the chaperone and Ubiquitin-proteasome system (6). Expression of murine Ataxin-3 is thought to be important for myogenesis, an effect that is dependent on the regulation of Integrin alpha 5 levels (7). Mutations in the ATXN3 gene are the cause of MJD, also known as spinocerebellar ataxia 3, an autosomal dominant neurodegenerative disorder characterized by nuclear aggregation of Ataxin-3 molecules featuring an expanded polyglutamine tract (2,8).
This recombinant protein is N-terminally tagged.
- Albrecht, M. et al. (2004) Eur. J. Biochem. 271:3155.
- Bettencourt, C. & M. Lima (2011) Orphanet. J. Rare Dis. 6:35.
- Rodrigues, A.J. et al. (2007) FASEB J. 21:1126.
- Todi, S.V. et al. (2009) EMBO J. 28:372.
- Scaglione, K.M. et al. (2011) Mol. Cell. 43:599.
- Connell, P. et al. (2001) Nat. Cell Biol. 3:93.
- do Carmo Costa, M. et al. (2001) PLoS One 5:e11728.
- Vo beta feldt, H. et al. (2012) PLoS One 7:e47452.
Citation for Recombinant Human His6-Ataxin-3 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
-
Mitochondrial Dysfunction in Spinocerebellar Ataxia Type 3 Is Linked to VDAC1 Deubiquitination
Authors: T Harmuth, JJ Weber, AJ Zimmer, AS Sowa, J Schmidt, JC Fitzgerald, L Schöls, O Riess, J Hübener-Sc
Oncogene, 2022-05-25;23(11):.
Species: Human
Sample Types: Proteins
Applications: Bioassay
FAQs
No product specific FAQs exist for this product, however you may
View all Proteins and Enzyme FAQsReviews for Recombinant Human His6-Ataxin-3 Protein, CF
Average Rating: 5 (Based on 2 Reviews)
Have you used Recombinant Human His6-Ataxin-3 Protein, CF?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by:
