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Recombinant Human His6-Josephin-1 Protein, CF

Catalog #: E-618 1 Review Datasheet / COA / SDS

Discontinued Product

E-618 has been discontinued.
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Recombinant Human His6-Josephin-1 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity
Recombinant Human Josephin-1 is a Ubiquitin-specific deconjugating enzyme.  Reaction Conditions will need to be optimized for each specific application.  We recommend an initial Josephin-1 concentration of 10-100 nM when using Ubiquitin-rhodamine (U-555) substrate and 100-500 nM when using Ubiquitin-AMC (U-550) substrate.  As supplied, this enzyme will not efficiently cleave purified recombinant polyubiquitin chains.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Josephin-1/JOSD1 protein
Contains a c-terminal 6His tag.
Accession #
Q15040
Predicted Molecular Mass
24 kDa

Product Datasheets

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E-618

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E-618

Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.0, 300 mM NaCl, 10% (v/v) Glycerol, 1 mM DTT
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 24 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Josephin-1/JOSD1

Josephin-1 is a member of the Machado-Joseph Disease (MJD) family of cysteine proteases, whose other members include Ataxin-3, Ataxin-3-like, and Josephin-2.  202 amino acids long with a predicted molecular weight of 23 kDa, Josephin-1 shares 97% identity with its mouse and rat orthologues.  When ubiquitinated, Josephin-1 is capable of cleaving K48- and K63-linked polyubiquitin chains in vitro.  Biologically, this enzyme may act to increase macropinocytosis and suppress clathrin- and caveolae-mediated endocytosis.

References
  1. Seki T. et al.  (2013) J. Biol. Chem.  288: 17145
  2. Weeks S.D. et al.  (2011) J. Biol. Chem.  286: 4555
Long Name
Josephin Domain Containing 1
Entrez Gene IDs
9929 (Human); 74158 (Mouse); 315134 (Rat)
Alternate Names
EC 3.4.19.12; JOSD1; Josephin domain containing 1; Josephin domain-containing 1; Josephin-1; JSPH1; KIAA0063dJ508I15.2

FAQs

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Reviews for Recombinant Human His6-Josephin-1 Protein, CF

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Recombinant Human His6-Josephin-1 Protein, CF
By Anonymous on 01/21/2021
Application: Enzymatic activity in vitro