Recombinant Human His6-UBE2E3 Protein, CF

Name: Recombinant Human His6-UBE2E3 Protein, CF
Brand: R&D Systems
SKU: E2-678
Price: 272 USD

Catalog #: E2-678
4 Citations Datasheet / COA / SDS

Discontinued Product

E2-678 has been discontinued.

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Citations (4)

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human His6-UBE2E3 Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Activity

Recombinant Human His6-Ube2E3 is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-Ube2E3 concentration of 0.1-1 μM.

Source

E. coli-derived human UBE2E3 protein
Contains an N-terminal 6-His tag

Accession #

NP_006348.1

Predicted Molecular Mass

25 kDa

Product Datasheets

E2-678

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E2-678

Formulation	X mg/ml (X μM) in 50 mM HEPES pH 8.0, 200 mM NaCl, 10% Glycerol (v/v), 1mM TCEP
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.

Reconstitution Calculator

Background: UBE2E3

Ubiquitin-conjugating Enzyme E2E 3 (UBE2E3), also known as Ubiquitin-conjugating Enzyme H9 (UbcH9), is a member of the Ubiquitin-conjugating (E2) enzyme family (1). UBE2E3 has a predicted molecular weight of 23 kDa. The human protein shares 100% amino acid sequence identity with the mouse and rat orthologs. UBE2E3 has an E2 catalytic core domain with an active site cysteine residue that is required for the formation of a thioester bond with Ubiquitin (2). This enzyme appears to be localized in the nucleus of interphase cells and cell-wide in mitotic cells (3). UBE2E3 may be regulated at the post-transcriptional level by miR-143 (4). The auto-ubiquitination of the ARA54, RNF8, and bacterially-encoded NleG Ubiquitin ligases (E3s) is mediated by UBE2E3 (5,6). UBE2E3 also functions with NEDD4L to regulate ENaC activity (7). Physiologically, UBE2E3 is required for the proliferation of retinal pigment epithelial cells via the negative regulation of p27/Kip1 levels (3). UBE2E3 may also be required for HIV-1 replication (8). This protein has an N-terminal His₆-tag. (Accession # NP_006348.1)

References

Ito, K. et al. (1999) Cytogenet. Cell Genet. 84:99.
Hershko, A. et al. (1983) J. Biol. Chem. 258:8206.
Plafker, K.S. et al. (2008) Invest. Ophthalmol. Vis. Sci. 49:5611.
Trakooljul, N. et al. (2010) Anim. Genet. 41:357.
Ito, K. et al. (2001) Eur. J. Biochem. 268:2725.
Wu, B. et al. (2010) PLoS Pathog. 6:e1000960.
Debonneville, C. et al. (2004) Mol. Cell. Biol. 24:2397.
Dziuba, N. et al. (2012) AIDS Res. Hum. Retroviruses 28:1329.

Entrez Gene IDs

10477 (Human); 22193 (Mouse); 295686 (Rat)

Alternate Names

E2-23K; EC 6.3.2.19; UBCE4; UbcH9; UBCH9ubiquitin-conjugating enzyme E2E 3 (homologous to yeast UBC4/5); UbcM2; UBE2E3; Ubiquitin carrier protein E3; ubiquitin-conjugating enzyme E2 E3; Ubiquitin-conjugating enzyme E2-23 kDa; ubiquitin-conjugating enzyme E2E 3 (UBC4/5 homolog, yeast); Ubiquitin-protein ligase E3

Related Research Areas

Citations for Recombinant Human His6-UBE2E3 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Targeting DCAF5 suppresses SMARCB1-mutant cancer by stabilizing SWI/SNF
Authors: Radko-Juettner, S;Yue, H;Myers, JA;Carter, RD;Robertson, AN;Mittal, P;Zhu, Z;Hansen, BS;Donovan, KA;Hunkeler, M;Rosikiewicz, W;Wu, Z;McReynolds, MG;Roy Burman, SS;Schmoker, AM;Mageed, N;Brown, SA;Mobley, RJ;Partridge, JF;Stewart, EA;Pruett-Miller, SM;Nabet, B;Peng, J;Gray, NS;Fischer, ES;Roberts, CWM;
Nature
Species: N/A
Sample Types: Recombinant Protein
Applications: Bioassay
Extracellular matrix stiffness determines DNA repair efficiency and cellular sensitivity to genotoxic agents
Authors: M Deng, J Lin, S Nowsheen, T Liu, Y Zhao, PW Villalta, D Sicard, DJ Tschumperl, S Lee, J Kim, Z Lou
Science Advances, 2020-09-11;6(37):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
CHIP ubiquitylates NOXA and induces its lysosomal degradation in response to DNA damage
Authors: MC Albert, K Brinkmann, W Pokrzywa, SD Günther, M Krönke, T Hoppe, H Kashkar
Cell Death & Disease, 2020-09-10;11(9):740.
Species: Human
Sample Types: Whole Cells
Applications: Ubiquitylation
A Virally Encoded DeSUMOylase Activity Is Required for Cytomegalovirus Reactivation from Latency
Authors: EL Poole, VG Kew, JCH Lau, MJ Murray, T Stamminger, JH Sinclair, MB Reeves
Cell Rep, 2018-07-17;24(3):594-606.
Applications: Bioassay