Recombinant Human His6-UBE2N/Ubc13 Protein, CF

Name: Recombinant Human His6-UBE2N/Ubc13 Protein, CF
Brand: R&D Systems
SKU: E2-660
Price: 286 USD

Catalog #: E2-660
2 Citations Datasheet / COA / SDS

Discontinued Product

E2-660 has been discontinued.

View all UBE2N/Ubc13 products.

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Citations (2)

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human His6-UBE2N/Ubc13 Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Activity

Recombinant Human His6-UBE2N/Ubc13 is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-UBE2N/Ubc13 concentration of 0.1-1 μM.

Source

E. coli-derived human UBE2N/Ubc13 protein
Contains a 6-His tag

Accession #

Q969M7

Predicted Molecular Mass

19 kDa

Product Datasheets

E2-660

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E2-660

Formulation	X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.

Reconstitution Calculator

Background: UBE2N/Ubc13

Ubiquitin-conjugating Enzyme E2N (UBE2N), also known as Ubiquitin-conjugating Enzyme 13 (Ubc13), is a member of the Ubiquitin-conjugating (E2) enzyme family. UBE2N/Ubc13 has a predicted molecular weight of 17 kDa. The human enzyme shares 100% and 99% amino acid sequence identity with its mouse and rat orthologs, respectively. UBE2N/Ubc13 has an E2 catalytic core domain with an active site cysteine residue that is required for the formation of a thioester bond with Ubiquitin (1). UBE2N/Ubc13 localizes to both the nucleus and cytoplasm (2). UBE2N/Ubc13 forms heterodimeric complexes with Uev1a/UBE2V1 and Mms2, both of which are catalytically inactive E2 enzyme variants. The UBE2N (Ubc13)/Uev1A Complex is found in the cytoplasm and is important for inflammatory responses via Nuclear Factor kappa B (NF-kappa B) activation (3,4). In contrast, the UBE2N/Ubc13-Mms2 complex functions in the nucleus and is required for an efficient DNA damage response (3,5-7). Pathologically, UBE2N (Ubc13)/Uev1a Complex-mediated NF-kappa B activation is required for the proliferation of diffuse large B-cell lymphoma cells (8). This protein has an N-terminal His₆-tag.

References

Hershko, A. et al. (1983) J. Biol. Chem. 258:8206.
Laine, A. et al. (2006) Mol. Cell. Biol. 26:8901.
Andersen, P.L. et al. (2005) J. Cell Biol. 170:745.
Pertel, T. et al. (2011) Nature 472:361.
Zhao, G.Y. et al. (2007) Mol. Cell 25:663.
Stewart, G.S. et al. (2009) Cell 136:420.
Campbell, S.J. et al. (2012) J. Biol. Chem. 287:23900.
Pulvino, M. et al. (2012) Blood 120:1668.

Entrez Gene IDs

7334 (Human); 93765 (Mouse); 116725 (Rat)

Alternate Names

bendless-like ubiquitin conjugating enzyme; Bendless-like ubiquitin-conjugating enzyme; BLU; EC 6.3.2.19; MGC131857; MGC8489; UBC13; UbcH13; UbcH-ben; UBE2N; Ubiquitin carrier protein N; ubiquitin-conjugating enzyme E2 N; ubiquitin-conjugating enzyme E2N (homologous to yeast UBC13); ubiquitin-conjugating enzyme E2N (UBC13 homolog, yeast); Ubiquitin-protein ligase N; yeast UBC13 homolog

Related Research Areas

Citations for Recombinant Human His6-UBE2N/Ubc13 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Targeting DCAF5 suppresses SMARCB1-mutant cancer by stabilizing SWI/SNF
Authors: Radko-Juettner, S;Yue, H;Myers, JA;Carter, RD;Robertson, AN;Mittal, P;Zhu, Z;Hansen, BS;Donovan, KA;Hunkeler, M;Rosikiewicz, W;Wu, Z;McReynolds, MG;Roy Burman, SS;Schmoker, AM;Mageed, N;Brown, SA;Mobley, RJ;Partridge, JF;Stewart, EA;Pruett-Miller, SM;Nabet, B;Peng, J;Gray, NS;Fischer, ES;Roberts, CWM;
Nature
Species: N/A
Sample Types: Recombinant Protein
Applications: Bioassay
Legionella pneumophila inhibits immune signalling via MavC-mediated transglutaminase-induced ubiquitination of UBE2N
Authors: N Gan, ES Nakayasu, PJ Hollenbeck, ZQ Luo
Nat Microbiol, 2018-11-12;4(1):134-143.
Species: Human
Sample Types: Protein
Applications: Bioassay