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Recombinant Human His6-UBE2N (Ubc13)/Uev1a Complex, CF

Catalog #: E2-664
17 Citations Datasheet / COA / SDS

Discontinued Product

E2-664 has been discontinued.
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Citations (17)
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Recombinant Human His6-UBE2N (Ubc13)/Uev1a Complex, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
Activity
Recombinant Human His6-UBE2N (Ubc13)/Uev1a Complex is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-UBE2N (Ubc13)/Uev1a Complex concentration of 0.1-1 μM.
Source
E. coli-derived human UBE2N (Ubc13)/Uev1a Complex protein
Contains an terminal 6-His tag
Accession #
P61088 (UBE2N) & Q13404 (Uev1a)
Predicted Molecular Mass

18 kDa (UBE2N) & 21 kDa (Uev1a)

Product Datasheets

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E2-664

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E2-664

Formulation

X mg/ml (X µM) in 50 mM HEPES pH 7.5, 200 mM Nacl, 10% Glycerol (v/v), 2 mM TCEP.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: UBE2N (Ubc13)/Uev1a Complex

Ubiquitin-conjugating Enzyme E2N (UBE2N), also known as Ubiquitin-conjugating Enzyme 13 (Ubc13), forms a functional complex with the catalytically inactive Uev1a/UBE2V1 protein (1). Human UBE2N/Ubc13 shares 100% and 99% amino acid (aa) sequence identity with the mouse and rat orthologs, respectively. Human Uev1a/UBE2V1 shares 89% aa sequence identity with its mouse and rat orthologs. The UBE2N (Ubc13)/Uev1a Complex functions with Ubiquitin ligases (E3s), including TRAF-6 and TRIM5, to synthesize Lys63-linked Ubiquitin chains (2,3). These Ubiquitin chains can either be unanchored or attached to target proteins (2,4). The UBE2N (Ubc13)/Uev1a Complex localizes primarily to the cytoplasm and is important for NF-kappa B activation (5). Additionally, this complex may function in the intracellular response to retroviral capsid recognition (3). Pathologically, the UBE2N (Ubc13)/Uev1a Complex is required for the proliferation of diffuse large B-cell lymphoma cells, suggesting that it may play a role in cancer (6).

References
  1. Deng, L. et al. (2000) Cell 103:351.
  2. Xia, Z.P. et al. (2009) Nature 461:114.
  3. Pertel, T. et al. (2011) Nature 472:361.
  4. Liu, C. et al. (2009) Sci. Signal. 2:ra63.
  5. Andersen, P.L. et al. (2005) J. Cell Biol. 170:745.
  6. Pulvino, M. et al. (2012) Blood 120:1668.
Entrez Gene IDs
7334 (Human)
Alternate Names
UBE2N (Ubc13)/Uev1a Complex

Citations for Recombinant Human His6-UBE2N (Ubc13)/Uev1a Complex, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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  1. TRIM21-dependent target protein ubiquitination mediates cell-free Trim-Away
    Authors: TET Mevissen, AV Prasad, JC Walter
    Cell Reports, 2023-02-18;42(2):112125.
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Bioassay
  2. Immune regulator LGP2 targets Ubc13/UBE2N to mediate widespread interference with K63 polyubiquitination and NF-kappaB activation
    Authors: JJ Lenoir, JP Parisien, CM Horvath
    Cell Reports, 2021-12-28;37(13):110175.
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Bioassay
  3. Extracellular matrix stiffness determines DNA repair efficiency and cellular sensitivity to genotoxic agents
    Authors: M Deng, J Lin, S Nowsheen, T Liu, Y Zhao, PW Villalta, D Sicard, DJ Tschumperl, S Lee, J Kim, Z Lou
    Science Advances, 2020-09-11;6(37):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. PHF7 Modulates BRDT Stability and Histone-to-Protamine Exchange during Spermiogenesis
    Authors: CR Kim, T Noda, H Kim, G Kim, S Park, Y Na, S Oura, K Shimada, I Bang, JY Ahn, YR Kim, SK Oh, HJ Choi, JS Kim, I Jung, H Lee, Y Okada, M Ikawa, SH Baek
    Cell Rep, 2020-07-28;32(4):107950.
    Species: Mouse
    Sample Types: Protein
    Applications: Bioassay
  5. Autophagy induction in atrophic muscle cells requires ULK1 activation by TRIM32 through unanchored K63-linked polyubiquitin chains
    Authors: M Di Rienzo, M Antonioli, C Fusco, Y Liu, M Mari, I Orhon, G Refolo, F Germani, M Corazzari, A Romagnoli, F Ciccosanti, B Mandriani, MT Pellico, R De La Torr, H Ding, M Dentice, M Neri, A Ferlini, F Reggiori, M Kulesz-Mar, M Piacentini, G Merla, GM Fimia
    Sci Adv, 2019-05-08;5(5):eaau8857.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Ubiquitination
  6. S-nitrosylation of cIAP1 switches cancer cell fate from TNF?/TNFR1-mediated cell survival to cell death
    Authors: S Romagny, S Bouaouiche, G Lucchi, P Ducoroy, JB Bertoldo, H Terenzi, A Bettaieb, S Plenchette
    Cancer Res., 2018-02-05;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  7. A20 regulates the DNA damage response and mediates tumor cell resistance to DNA damaging therapy
    Authors: C Yang, W Zang, Z Tang, Y Ji, R Xu, Y Yang, A Luo, B Hu, Z Zhang, Z Liu, X Zheng
    Cancer Res., 2017-12-12;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  8. A ubiquitin-dependent signalling axis specific for ALKBH-mediated DNA dealkylation repair
    Authors: JR Brickner, JM Soll, PM Lombardi, CB Vågbø, MC Mudge, C Oyeniran, R Rabe, J Jackson, ME Sullender, E Blazosky, AK Byrum, Y Zhao, MA Corbett, J Gécz, M Field, A Vindigni, G Slupphaug, C Wolberger, N Mosammapar
    Nature, 2017-11-08;551(7680):389-393.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  9. Internally tagged ubiquitin: a tool to identify linear polyubiquitin-modified proteins by mass spectrometry
    Authors: K Kliza, C Taumer, I Pinzuti, M Franz-Wach, S Kunzelmann, B Stieglitz, B Macek, K Husnjak
    Nat. Methods, 2017-03-20;0(0):.
    Applications: Bioassay
  10. TRIM65-catalized ubiquitination is essential for MDA5-mediated antiviral innate immunity
    Authors: Xueting Lang
    J. Exp. Med, 2016-12-28;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  11. Cell cycle-regulated ubiquitination of tankyrase 1 by RNF8 and ABRO1/BRCC36 controls the timing of sister telomere resolution
    Authors: Ekta Tripathi
    EMBO J, 2016-12-19;0(0):.
    Applications: Bioassay
  12. SKP2 loss destabilizes EZH2 by promoting TRAF6-mediated ubiquitination to suppress prostate cancer
    Oncogene, 2016-11-21;0(0):.
    Applications: Bioassay
  13. TRAF6 Restricts p53 Mitochondrial Translocation, Apoptosis, and Tumor Suppression
    Authors: Hui-Kuan Lin
    Mol. Cell, 2016-11-03;64(4):803-814.
    Applications: Bioassay
  14. FBXW7 Facilitates Nonhomologous End-Joining via K63-Linked Polyubiquitylation of XRCC4
    Authors: Q Zhang, D Karnak, M Tan, TS Lawrence, MA Morgan, Y Sun
    Mol. Cell, 2016-01-07;61(3):419-33.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  15. ASH2L regulates ubiquitylation signaling to MLL: trans-regulation of H3 K4 methylation in higher eukaryotes.
    Authors: Wu L, Lee S, Zhou B, Nguyen U, Muir T, Tan S, Dou Y
    Mol Cell, 2013-02-28;49(6):1108-20.
    Applications: Bioassay
  16. The E3 ubiquitin ligases RNF126 and Rabring7 regulate endosomal sorting of the epidermal growth factor receptor.
    Authors: Smith, Christop, Berry, Donna M, McGlade, C Jane
    J Cell Sci, 2013-02-15;126(0):1366-80.
    Applications: Bioassay
  17. p47 negatively regulates IKK activation by inducing the lysosomal degradation of polyubiquitinated NEMO.
    Authors: Shibata Y, Oyama M, Kozuka-Hata H, Han X, Tanaka Y, Gohda J, Inoue J
    Nat Commun, 2012-01-01;3(0):1061.
    Applications: Binding Assay

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