Recombinant Human His6-USP11 Protein, CF

Name: Recombinant Human His6-USP11 Protein, CF
Brand: R&D Systems
SKU: E-602
Price: 323 USD

Catalog #: E-602 Datasheet / COA / SDS

Discontinued Product

E-602 has been discontinued.

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Recombinant Human His6-USP11 Protein, CF Summary

Product Specifications

Purity

>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Activity

Reaction conditions will need to be optimized for each specific application. We recommend an initial recombinant human USP11 concentration of 10-50 nM when using Ubiquitin-AMC or Ubiquitin-Rhodamine substrates (U-550, U-555).

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human USP11 protein
Contains an C-terminal 6-His tag

Accession #

P51784

Predicted Molecular Mass

111 kDa

Product Datasheets

E-602

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E-602

Formulation	X mg/ml (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 10% (v/v) Glycerol, 1 mM TCEP
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.

Reconstitution Calculator

Background: USP11

Ubiquitin carboxyl-terminal hydrolase 11 (USP11) is a specialized cysteine protease with a predicted molecular weight of 110 kDa. USP11 is a member of the peptidase C19 family and the human protein shares 77% amino acid sequence identity with its mouse ortholog. USP11 plays a role in DNA double-strand break (DSB) repair, and cells are hypersensitive to gamma radiation when USP11 has been knocked down. USP11 overexpression is often observed in colorectal cancer and melanoma and is correlated with poor survival. In these cases USP11 may be driving disease states by stabilizing cIAP2. Interestingly, recent genetic analysis suggests that USP11 was generated during the course of vertebrate evolution by a small-scale duplication of the USP4-encoding region. This recombinant protein contains a C-terminal 6-His tag.

References

Lee, E.W. et al. (2015) Cell Death and Differ. 22: 1463
Vlasschaert, C. et al. (2015) BMC Evol. Biol. 15: 230
Yu, M. et al. (2016) J.Biol.Chem. 291: 959

Long Name

Ubiquitin Specific Protease 11

Entrez Gene IDs

8237 (Human); 236733 (Mouse); 408217 (Rat)

Alternate Names

Deubiquitinating enzyme 11; EC 3.1.2.15; EC 3.4.19.12; ubiquitin carboxyl-terminal hydrolase 11; ubiquitin specific peptidase 11; ubiquitin specific protease 11; Ubiquitin Thioesterase 11; Ubiquitin thiolesterase 11; ubiquitin-specific processing protease 11; Ubiquitin-specific-processing protease 11; UHX1; UHX1ubiquitin carboxyl-terminal hydrolase, X-linked; USP11