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Recombinant Human His6-USP13 Protein, CF

Catalog #: E-588
3 Citations Datasheet / COA / SDS

Discontinued Product

E-588 has been discontinued.
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Recombinant Human His6-USP13 Protein, CF Summary

Product Specifications

Purity
>98%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
Activity

Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human USP13 concentration of 100-500 nM when using Ubiquitin-AMC or Ubiquitin-Rh110 substrates (U-550, U-555)

Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human USP13 protein
Contains an C-terminal 6-His tag
Accession #
Q92995
Predicted Molecular Mass
98 kDa

Product Datasheets

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E-588

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E-588

Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 10% (v/v) Glycerol, 1 mM TCEP
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: USP13

Ubiquitin carboxyl-terminal hydrolase 13 (USP13) is a specialized cysteine protease with a predicted molecular weight of 97 kDa. USP13 is a member of the peptidase C19 family and the human protein shares 96% amino acid sequence identity with its mouse ortholog. Recombinant USP13 has weak deubiquitinase activity against unanchored polyubiquitin chains, but will disassemble tetraubiquitin chains in extended reactions, particularly K63 and K48 linkages.  Suggested biological targets of USP13 include PTEN, BECN1, MITF, SKP2 and USP10, and roles for USP13 in melanoma and colorectal cancer have been reported. This recombinant protein contains a C-terminal 6-His tag.

References
  1. Xiang, S. et al. (2015) Oncol Rep. 33: 1342
  2. Zhang, J. et al. (2013) Nat Cell Biol. 15: 1486
  3. Zhang, Y-H. et al. (2011) PLoS One 6: e29362
  4. Zhao, X. et al. (2011) Nat Commun. 2: 414. doi: 10.1038/ncomms1421
Long Name
Ubiquitin Specific Protease 13
Entrez Gene IDs
8975 (Human); 72607 (Mouse); 310306 (Rat)
Alternate Names
Deubiquitinating enzyme 13; EC 3.1.2.15; EC 3.4.19.12; Isopeptidase T-3; IsoT-3; ISOT3IsoT-3; ubiquitin carboxyl-terminal hydrolase 13; ubiquitin specific peptidase 13 (isopeptidase T-3); ubiquitin specific protease 13 (isopeptidase T-3); Ubiquitin Thioesterase 13; Ubiquitin thiolesterase 13; Ubiquitin-specific-processing protease 13; USP13

Citations for Recombinant Human His6-USP13 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Site-specific ubiquitylation acts as a regulator of linker histone H1
    Authors: E Höllmüller, S Geigges, ML Niedermeie, KM Kammer, SM Kienle, D Rösner, M Scheffner, A Marx, F Stengel
    Nature Communications, 2021-06-09;12(1):3497.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  2. The ubiquitin isopeptidase USP10 deubiquitinates LC3B to increase LC3B levels and autophagic activity
    Authors: R Jia, JS Bonifacino
    The Journal of Biological Chemistry, 2021-02-10;0(0):100405.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Deubiquitination
  3. Deubiquitinase USP13 dictates MCL1 stability and sensitivity to BH3 mimetic inhibitors
    Authors: S Zhang, M Zhang, Y Jing, X Yin, P Ma, Z Zhang, X Wang, W Di, G Zhuang
    Nat Commun, 2018-01-15;9(1):215.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay

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