Home / ZRANB1/Trabid / Recombinant Human His6-ZRANB1/Trabid Protein, CF

Recombinant Human His6-ZRANB1/Trabid Protein, CF

Catalog #: E-560
2 Citations Datasheet / COA / SDS

Discontinued Product

E-560 has been discontinued.
View all ZRANB1/Trabid products.
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Citations (2)
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Recombinant Human His6-ZRANB1/Trabid Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
Activity
Recombinant Human His6-ZRANB1/Trabid is a Ubiquitin-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-ZRANB1/Trabid concentration of 0.1-1 μM.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human ZRANB1/Trabid protein
Contains a C-terminal 6-His tag
Accession #
Q9UGI0
Predicted Molecular Mass
82 kDa

Product Datasheets

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E-560

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E-560

Formulation

X mg/ml (X μM) in 50 mM HEPES pH 8.0, 100 mM NaCl, 1 mM TCEP
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ZRANB1/Trabid

Zinc Finger, RAN-binding Domain Containing 1 (ZRANB1), also known as TRAF-binding Domain-containing Protein (Trabid), is a C64 type peptidase and a member of the ovarian tumor (OTU) protein super-family with a predicted molecular weight of 81 kDa (1). The human protein shares 99% amino acid sequence identity with its mouse ortholog. ZRANB1 preferentially cleaves K29-, K33-, and K63-linked poly-Ubiquitin chains (2). It has been shown to play a role in the regulation of Wnt signaling via deubiquitination of APC (3,4). This recombinant protein contains a C-terminal 6-His tag.

References
  1. Evans, P.C. et al. (2001) Biochem. J. 357:617.
  2. Licchesi, J.D. et al. (2011) Nat. Struct. Mol. Biol. 19:62.
  3. Tran, H. et al. (2008) Genes Dev. 22:528.
  4. Shi, T. et al. (2012) BMC Chem. Biol. 12:4.
    5.  
Long Name
Zinc Finger, RAN-binding Domain Containing 1
Entrez Gene IDs
54764 (Human); 360216 (Mouse)
Alternate Names
HRPE773; jacalin-like lectin domain containing 2; JCLN2; pancreatic adenocarcinoma upregulated factor; PAUF; PRO1567; Trabid; ZRANB1; zymogen granule protein 16 homolog B (rat); zymogen granule protein 16 homolog B

Citations for Recombinant Human His6-ZRANB1/Trabid Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. TRABID inhibition activates cGAS/STING-mediated anti-tumor immunity through mitosis and autophagy dysregulation
    Authors: Chen, YH;Chen, HH;Wang, WJ;Chen, HY;Huang, WS;Kao, CH;Lee, SR;Yeat, NY;Yan, RL;Chan, SJ;Wu, KP;Chen, RH;
    Nature communications
    Species: N/A
    Sample Types: Proteins
    Applications: Bioassay
  2. VPS34 K29/K48 branched ubiquitination governed by UBE3C and TRABID regulates autophagy, proteostasis and liver metabolism
    Authors: YH Chen, TY Huang, YT Lin, SY Lin, WH Li, HJ Hsiao, RL Yan, HW Tang, ZQ Shen, GC Chen, KP Wu, TF Tsai, RH Chen
    Nature Communications, 2021-02-26;12(1):1322.
    Species: Human
    Sample Types: Whole Cells
    Applications: Ubiquitination

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