Recombinant Human His6-ZRANB1/Trabid Protein, CF

R&D Systems | Catalog # E-560

R&D Systems
Discontinued Product
E-560 has been discontinued. View all ZRANB1/Trabid products.

Key Product Details

Source

Sf 21 (baculovirus)

Accession Number

Q9UGI0

Applications

Enzyme Activity
View all ZRANB1/Trabid Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human ZRANB1/Trabid protein
Contains a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

82 kDa

Activity

Recombinant Human His6-ZRANB1/Trabid is a Ubiquitin-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-ZRANB1/Trabid concentration of 0.1-1 μM.

Formulation, Preparation, and Storage

E-560
Formulation

X mg/ml (X μM) in 50 mM HEPES pH 8.0, 100 mM NaCl, 1 mM TCEP
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: ZRANB1/Trabid

Zinc Finger, RAN-binding Domain Containing 1 (ZRANB1), also known as TRAF-binding Domain-containing Protein (Trabid), is a C64 type peptidase and a member of the ovarian tumor (OTU) protein super-family with a predicted molecular weight of 81 kDa (1). The human protein shares 99% amino acid sequence identity with its mouse ortholog. ZRANB1 preferentially cleaves K29-, K33-, and K63-linked poly-Ubiquitin chains (2). It has been shown to play a role in the regulation of Wnt signaling via deubiquitination of APC (3,4). This recombinant protein contains a C-terminal 6-His tag.

References

  1. Evans, P.C. et al. (2001) Biochem. J. 357:617.
  2. Licchesi, J.D. et al. (2011) Nat. Struct. Mol. Biol. 19:62.
  3. Tran, H. et al. (2008) Genes Dev. 22:528.
  4. Shi, T. et al. (2012) BMC Chem. Biol. 12:4.
    5.  

Long Name

Zinc Finger, RAN-binding Domain Containing 1

Alternate Names

Trabid

Entrez Gene IDs

54764 (Human); 360216 (Mouse)

Gene Symbol

ZRANB1

UniProt

Q9UGI0 (Human)

Additional ZRANB1/Trabid Products

Product Documents for Recombinant Human His6-ZRANB1/Trabid Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Human His6-ZRANB1/Trabid Protein, CF

For research use only

Citations for Recombinant Human His6-ZRANB1/Trabid Protein, CF

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FAQs

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Associated Pathways

Ubiquitination Cascade Pathway Ubiquitination Cascade Pathway Thumbnail