Recombinant Human IgE Fc His-tag Protein, CF New

Catalog #: 11647-IE Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
11647-IE-100

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Recombinant Human IgE Fc His-tag Protein Binding Activity.

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human IgE Fc His-tag Protein, CF Summary

R&D Systems CHO-derived Recombinant Human IgE Fc His-tag Protein (11647-IE)
Quality control testing to verify active proteins with lot specific assays by in-house scientists
All R&D Systems proteins are covered with a 100% guarantee

Product Specifications

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. Recombinant Human IgE Fc His-tag binds to Recombinant Human Fc epsilon RI alpha Fc Chimera Protein (Catalog # 11561-FC) with an ED₅₀ of 0.100-1.00 ng/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human IgE Fc protein
Ser106-Lys428, with an N-terminal 6-His tag

Accession #

P01854-1

N-terminal Sequence
Analysis

His

Structure / Form

Disulfide linked homodimer

Predicted Molecular Mass

37 kDa

SDS-PAGE

40-56 kDa, under reducing conditions

Product Datasheets

11647-IE

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11647-IE

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 250 μg/mL in water.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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Measured by its binding ability in a functional ELISA. Recombinant Human IgE Fc His-tag Protein (Catalog # 11647-IE) binds to Recombinant Human Fc epsilon RI alpha Fc Chimera Protein ( 11561-FC) with an ED₅₀ of 0.100-1.00 ng/mL.

SDS-PAGE

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2 μg/lane of Recombinant Human IgE Fc His-tag Protein (Catalog # 11647-IE) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 40‑56 kDa, under reducing conditions.

Reconstitution Calculator

Background: IgE Fc

IgE is an immunoglobulin that plays a critical role in the immune response to parasitic infections and in the development of allergic reactions. Like other antibody classes, IgE has the same molecular architecture that includes two heavy chains and two light chains, but its heavy chain contains an additional domain compared to IgG heavy chain. IgE binds to its receptor, Fc epsilon RI, on the surface of mast cells and basophils, triggering the release of histamine and other mediators that cause the symptoms of allergic reactions (1, 2). The recombinant human IgE-Fc protein is a valuable tool for researchers studying the mechanisms of allergic reactions and the development of new therapies for allergic diseases. It can be used in a wide range of applications, including ELISA assays, Western blotting, functional assays to study the binding of IgE to its receptor, Fc epsilon RI, and the downstream signaling pathways that are activated upon binding (1, 2, 3). In addition to its research applications, the recombinant human IgE-Fc protein has potential therapeutic applications in the treatment of allergic diseases. Therapies that target IgE have been shown to be effective in the treatment of allergic diseases such as asthma and atopic dermatitis (4).

References