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Recombinant Human IL-18/IL-1F4 Protein

R&D Systems | Catalog # 9124-IL

Analyzed by SEC-MALS
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Key Product Details

  • R&D Systems E. coli-derived Recombinant Human IL-18/IL-1F4 Protein (9124-IL)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Accession Number

Q14116

Applications

Bioactivity
View all IL-18/IL-1F4 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human IL-18/IL-1F4 protein
Tyr37-Asp193

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Tyr37

Predicted Molecular Mass

18 kDa

SDS-PAGE

18 kDa, reducing conditions

Activity

Measured by its ability to induce IFN-gamma secretion by KG‑1 human acute myelogenous leukemia cells in the presence of TNF-alpha.
The ED50 for this effect is 1.5-9 ng/mL.

Reviewed Applications

Read 11 reviews rated 4.6 using 9124-IL in the following applications:

Scientific Data Images for Recombinant Human IL-18/IL-1F4 Protein

Recombinant Human IL‑18/IL‑1F4 Protein SEC-MALS.

Recombinant Human IL-18 (Catalog # 9124-IL) has a molecular weight (MW) of 17.5 kDa as analyzed by SEC-MALS, suggesting that this protein is a monomer.
Recombinant Human IL-18/IL-1F4 Protein Bioactivity

Recombinant Human IL-18/IL-1F4 Protein Bioactivity

Recombinant Human IL-18 (Catalog # 9124-IL) induces IFN-gamma secretion by KG‑1 human acute myelogenous leukemia cells. The ED50 for this effect is 1.5-9 ng/mL.
Recombinant Human IL-18/IL-1F4 Protein SDS-PAGE

Recombinant Human IL-18/IL-1F4 Protein SDS-PAGE

1 μg/lane of Recombinant Human IL-18/IL-1F4 (Catalog # 9124-IL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing a single band at 18 kDa.

Formulation, Preparation, and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 9124-IL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and DTT with BSA as a carrier protein.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 9124-IL/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and DTT.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-18/IL-1F4

Interleukin-18 (IL-18) is a proinflammatory cytokine in the IL-1 family that exerts distinct immune effects depending on the local cytokine environment. It is expressed as a 24 kDa precursor by endothelial and epithelial cells, keratinocytes, gamma delta  T cells, and phagocytes. The precursor is activated intracellularly by Caspase-1 mediated proteolysis to release the 17 kDa mature cytokine. The precursor can also be released by necrotic cells for extracellular cleavage by multiple proteases.
IL-18 activation is induced by infection or tissue damage and contributes to disease pathology in chronic inflammation (1-3). IL-18 binds to the widely expressed
IL-18 R alpha which recruits IL-18 R beta to form the signaling receptor complex (4, 5). Its bioactivity is negatively regulated by interactions with IL-18 binding proteins and virally encoded IL-18BP homologs (6). In the presence of IL-12 or IL-15, IL-18 enhances anti-viral Th1 immune responses by inducing IFN-gamma production and the cytolytic activity of CD8+ T cells and NK cells (7, 8). In the absence of IL-12 or IL-15, however, IL-18 promotes production of the Th2 cytokines IL-4 and IL-13 by CD4+ T cells and basophils (9, 10). In the presence of IL-1 beta or IL-23, IL-18 induces the antigen-independent production of IL-17 by gamma delta  T cells and CD4+ T cells (11).
IL-18 also promotes myeloid dendritic cell maturation and triggers neutrophil respiratory burst (12, 13). In cancer, IL-18 exhibits diverse activities including enhancing anti-tumor immunity, inhibiting or promoting angiogenesis, and promoting tumor cell metastasis (14). Mature human IL-18 shares approximately 63% amino acid sequence identity with mouse and rat IL-18 (15). Alternative splicing in human ovarian cancer generates an isoform that is resistant to Caspase-1 activation (16). A cell surface form can be expressed on M-CSF induced macrophages and released in response to bacterial endotoxin (17).

References

  1. Dinarello, C.A. et al. (2013) Front. Immunol. 4:289.
  2. Smith, D.E. (2011) J. Leukoc. Biol. 89:383.
  3. Gu, Y. et al. (1997) Science 275:206.
  4. Torigoe, K. et al. (1997) J. Biol. Chem. 272:25737.
  5. Cheung, H. et al. (2005) J. Immunol. 174:5351.
  6. Novick, D. et al. (1999) Immunity 10:127.
  7. Fehniger, T.A. et al. (1999) J. Immunol. 162:4511.
  8. Yoshimoto, T. et al. (1998) J. Immunol. 161:3400.
  9. Yoshimoto, T. et al. (2000) Nat. Immunol. 1:132.
  10. Kroeger, K.M. et al. (2009) J. Leukoc. Biol. 86:769.
  11. Lalor, S.J. et al. (2011) J. Immunol. 186:5738.
  12. Li, J. et al. (2004) Cell. Immunol. 227:103.
  13. Elbim, C. et al. (2005) Clin. Diagn. Lab. Immunol. 12:436.
  14. Fabbi, M. et al. (2015) J. Leukoc. Biol. 97:665.
  15. Ushio, S. et al. (1996) J. Immunol. 156:4274.
  16. Gaggero, A. et al. (2004) Oncogene 23:7552.
  17. Bellora, F. et al. (2012) Eur. J. Immunol. 42:1618.

Long Name

Interleukin 18

Alternate Names

IGIF, IL-1F4, IL-1g, IL18

Entrez Gene IDs

3606 (Human); 16173 (Mouse); 29197 (Rat); 397057 (Porcine); 574151 (Primate)

Gene Symbol

IL18

UniProt

Q14116

Additional IL-18/IL-1F4 Products

Product Documents for Recombinant Human IL-18/IL-1F4 Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 9124-IL (with carrier)
Download SDS 9124-IL/CF (carrier free)

Product Specific Notices for Recombinant Human IL-18/IL-1F4 Protein

For research use only

Citations for Recombinant Human IL-18/IL-1F4 Protein

Customer Reviews for Recombinant Human IL-18/IL-1F4 Protein (11)

4.6 out of 5
11 Customer Ratings
5 Stars
64%
4 Stars
36%
3 Stars
0%
2 Stars
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1 Stars
0%

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Showing  1 - 5 的 11 reviews Showing All
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  • Recombinant Human IL-18/IL-1F4 Protein
    Name: Anonymous
    Application: Immunoassay Standard
    Verified Customer | Posted 07/24/2023
    Recombinant Human IL-18/IL-1F4 Protein 9124-IL
  • Recombinant Human IL-18/IL-1F4 Protein
    Name: Anonymous
    Application: Tumor Spheroid kill
    Verified Customer | Posted 02/02/2023
    Recombinant Human IL-18/IL-1F4 Protein 9124-IL
  • Recombinant Human IL-18/IL-1F4 Protein
    Name: Lamberto Torralba
    Application: qPCR
    Verified Customer | Posted 09/23/2022
    Target protein upregulation after IL-18 treatment (PBMCs, 18h)
    Recombinant Human IL-18/IL-1F4 Protein 9124-IL
  • Recombinant Human IL-18/IL-1F4 Protein
    Name: Lamberto Torralba
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 09/23/2022
    Contribution of IL-18 to intracellular pro-inflammatory cytokine production in human CD8+ T cells
    Recombinant Human IL-18/IL-1F4 Protein 9124-IL
  • Recombinant Human IL-18/IL-1F4 Protein
    Name: Lamberto Torralba
    Application: SDS-PAGE Control
    Verified Customer | Posted 09/23/2022
    Target protein upregulation in primary NK cells upon IL-18 stimulation
    Recombinant Human IL-18/IL-1F4 Protein 9124-IL
  • Recombinant Human IL-18/IL-1F4 Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 10/26/2020
    I am looking at the inhibition of IFN-r production by the compound with the presence of IL23 and IL-18.
    Recombinant Human IL-18/IL-1F4 Protein 9124-IL
  • Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 10/08/2018
  • Recombinant Human IL-18/IL-1F4 Protein
    Name: Anonymous
    Application: Enzymatic activity in vitro
    Verified Customer | Posted 08/06/2018
  • Recombinant Human IL-18/IL-1F4 Protein
    Name: Greta Beckerle
    Application: Media additive for protein or antibody production
    Verified Customer | Posted 12/22/2017
  • Recombinant Human IL-18/IL-1F4 Protein
    Name: Anonymous
    Application: Immunoassay Standard
    Verified Customer | Posted 12/07/2017
    Performed similarly to Competitor1 and Competitor2 proteins in immunoassay
  • Recombinant Human IL-18/IL-1F4 Protein
    Name: Greta Beckerle
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 12/04/2017

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FAQs for Recombinant Human IL-18/IL-1F4 Protein

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    • Q: What is the difference between the two versions of Recombinant Human IL-18, Catalog # 9124-IL and Catalog # B001-5?
      A: Catalog # 9124-IL is manufactured by R&D Systems, while Catalog # B001-5 is manufactured by MBL.  Catalog # 9124-IL is lyophilized powder whereas Catalog # B001-5 is supplied in solution (PBS with BSA and sucrose). Activity of the two proteins is measured in similar cell-based assays; please consult the respective product-specific pages for assay details.  Catalog # 9124-IL is competitively priced and has higher purity and lower endotoxin than Catalog # B001-5.
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