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Recombinant Human IL-27 Protein

R&D Systems | Catalog # 2526-IL

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Key Product Details

  • R&D Systems NS0-derived Recombinant Human IL-27 Protein (2526-IL)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

Q14213.2 (EBI3) & AAM34498 (p28)

Applications

Bioactivity
View all IL-27 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human IL-27 protein
Human IL-27 EBI-3
(Arg21-Lys229)
Accession # Q14213.2		 GSGSSRGGSGSGGSGGGGSKL Human IL-27 p28
(Phe29-Pro243)
Accession # AAM34498		 6-His tag
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Arg21

Predicted Molecular Mass

50 kDa

SDS-PAGE

60 kDa, under reducing conditions.

Activity

Measured in an anti-viral assay using HepG2 human hepatocellular carcinoma cells infected with encephalomyocarditis (EMC) virus. Bender H. et al. (2009) Hepatology 50:585.
The ED50 for this effect is 1-8 ng/mL.

Reviewed Applications

Read 4 reviews rated 4.5 using 2526-IL in the following applications:

Formulation, Preparation, and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 2526-IL
Formulation Lyophilized from a 0.2 μm filtered solution in HEPES, NaCl, EDTA and CHAPS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 2526-IL/CF
Formulation Lyophilized from a 0.2 μm filtered solution in HEPES, NaCl, EDTA and CHAPS with Trehalose.
Reconstitution Reconstitute at 100 μg/mL in sterile, deionized water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-27

IL-27 is a heterodimeric group 2 receptor ligand molecule that belongs to the IL-6/IL-12 family of long type I cytokines (1). It is composed of EBI3 (EBV-induced gene 3), a 34 kDa glycoprotein that is related to the p40 subunit of IL-12 and IL-23, and p28, the 28 kDa glycoprotein that is related to the p35 chain of IL-12 (2-4). The human EBI3 gene encodes a 229 amino acid (aa) precursor that contains a 20 aa signal peptide and 209 aa mature protein (5). The mature region contains two potential N-linked glycosylation sites, two fibronectin type III domains, and two pairs of conserved cysteine residues with a WSXWS-like motif that places the molecule in the hematopoietin receptor family (5). Although p40, the EBI3 counterpart in IL-12, is known to form homodimers, there is no evidence to date that EBI3 also homodimerizes. Human EBI3 is 61% aa identical to mouse EBI3. The human p28 gene encodes a 243 aa precursor that contains a 28 aa signal sequence and 215 aa mature region (6). The mature region is characterized by the presence of four alpha -helices, placing it in the IL-6 family of helical cytokines. Human p28 is 74% aa identical to mouse p28. IL-27 is expressed by monocytes, endothelial cells and dendritic cells (7). IL-27 binds to and signals through a heterodimeric receptor complex composed of WSX-1 (TCCR) and gp130. Evidence suggests IL-27 interacts only with WSX-1 (6, 8, 9). IL-27 has both anti- and proinflammatory properties. As an anti-inflammatory, IL-27 seems to induce a general negative feedback program that limits T and NK-T cell activity (3, 7). At the onset of infection, IL-27 induces an IL-12 receptor on naïve CD4+ T cells, making them susceptible to subsequent IL-12 activity (and possible Th1 development) (10).

References

  1. Boulay, J-L. et al. (2003) Immunity 19:159. 
  2. Trinchieri, G. et al. (2003) Immunity 19:641. 
  3. Murakami, M. et al. (2004) Growth Factors 22:75. 
  4. Cordoba-Rodriguez, R. and D.M. Frucht (2003) Exp. Opin. Biol. Ther. 3:715. 
  5. Devergne, O. et al. (1996) J. Virology 70:1143. 
  6. Pflanz, S. et al. (2002) Immunity 16:779.
  7. Villarino, A.V. et al. (2004) J. Immunol. 173:715.
  8. Pflanz, S. et al. (2004) J Immunol 172:2225.
  9. Scheller, J. et al. (2005) Biochem. Biophys. Res. Commun. 326:724.
  10. Holscher, C. (2004) Med. Microbiol. Immunol. (Berl).193:1.

Long Name

Interleukin 27

Alternate Names

IL27

Entrez Gene IDs

246778 (Human); 246779 (Mouse); 102121242 (Cynomolgus Monkey)

Gene Symbol

IL27

UniProt

Q14213.2 (EBI3) & AAM34498 (p28)

Additional IL-27 Products

Product Documents for Recombinant Human IL-27 Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 2526-IL (with carrier)
Download SDS 2526-IL/CF (carrier free)

Product Specific Notices for Recombinant Human IL-27 Protein

For research use only

Citations for Recombinant Human IL-27 Protein

Customer Reviews for Recombinant Human IL-27 Protein (4)

4.5 out of 5
4 Customer Ratings
5 Stars
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4 Stars
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1 Stars
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  • Recombinant Human IL-27 Protein
    Name: Anonymous
    Application: Stem/Immune cell maintenance or differentiation
    Verified Customer | Posted 06/16/2020
  • Recombinant Human IL-27 Protein
    Name: Anonymous
    Application: Immunoassay Standard
    Verified Customer | Posted 12/02/2019
    Recombinant Human IL-27 Protein 2526-IL
  • Name: Anonymous
    Application: Stem/Immune cell maintenance or differentiation
    Verified Customer | Posted 03/28/2018
  • Recombinant Human IL-27 Protein
    Name: Anonymous
    Application: Immunoassay Standard
    Verified Customer | Posted 11/27/2017
    AF2526 was used as the solid phase antibody, BAF2526 was used as the detection antibody. 2526-IL was used as the immunoassay standard. Sensitivity of the assay was around 100 pg/ml.
    Recombinant Human IL-27 Protein 2526-IL

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