Recombinant Human Keratocan His-tag Protein, CF

Catalog #: 10838-KE Datasheet / COA / SDS

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10838-KE-050

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Recombinant Human Keratocan His-tag Protein SDS-PAGE.

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Keratocan His-tag Protein, CF Summary

R&D Systems CHO-derived Recombinant Human Keratocan His-tag Protein (10838-KE)
Quality control testing to verify active proteins with lot specific assays by in-house scientists
All R&D Systems proteins are covered with a 100% guarantee

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human Keratocan His-tag is immobilized at 1.00 μg/mL (100 µL/well), Recombinant Human BLMH/Bleomycin Hydrolase (Catalog # 6200-CY) binds with an ED₅₀ of 1.20-12.0 μg/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human Keratocan protein
Arg21-Ile352, with a C-terminal 6-His tag

Accession #

O60938.1

N-terminal Sequence
Analysis

Arg21

Predicted Molecular Mass

40 kDa

SDS-PAGE

44-62 kDa, under reducing conditions

Product Datasheets

10838-KE

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10838-KE

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 1 mg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

SDS-PAGE

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2 μg/lane of Recombinant Human Keratocan His-tag (Catalog # 10838-KE) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 44-62 kDa.

Reconstitution Calculator

Background: Keratocan

Keratocan is a keratan sulfate-containing proteoglycan that is highly expressed in human cornea (1, 2). It is an extracellular matrix protein that belongs to the class II small leucine-rich proteoglycan family (1). This class of protein contains 10-12 leucine-rich repeat motifs flanked by conserved cysteine residues in N-and C-terminal domains of the core proteins (1, 3). Human keratocan is expressed as a 352 amino acid (aa) protein that includes a 20 aa signal peptide and the 332 aa keratocan protein containing ten LRR motifs (2). Human keratocan shares 87% and 88% aa identity with mouse and rat keratocan, respectively. Keratocan is expressed in the cornea and tendon where it is likely to play a role in collagen fibrillogenesis and in maintaining corneal clarity (4, 5). Keratocan is also expressed in the bone and can modulate osteogenic differentiation (6).

References