Recombinant Human LTK Protein, CF
Recombinant Human LTK Protein, CF Summary
Ser20-Pro424, with a C-terminal His-tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 500 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
When Recombinant Human LTK (Catalog # 9376-LK) is immobilized at 2 μg/mL, 100 μL/well, Recombinant Human FAM-150A binds with an ED50 of 40-240 ng/mL.
LTK, also known as Leukocyte tyrosine kinase receptor and protein tyrosine kinase 1, is a type I transmembrane protein and shares a high degree of sequence similarity with kinases in the insulin receptor family (1). Alternative splicing generates several isoforms of 50-100 kDa (2). LTK contains a 408 aa extracellular domain, 25 aa transmembrane domain, and 415 aa cytoplasmic domain. The cytoplasmic region has several phosphotyrosines that interact with downstream signaling molecules (3). LTK is reported to undergo dimerand trimerization when under certain conditions. The extracellular domain of human LTK shares 75% homology with mouse LTK. It is expressed in preB lymphocytes, B lymphocytes, and other hematopoietic cells, as well as brain and placenta (48). Human LTK can bind to PLCgamma 1, PI3K, GAP and Raf1 in vivo (6). LTK promotes neurite outgrowth via PI3K/AKT and Ras/MAPK pathways (7). FAM150A and FAM150B can both bind to the extracellular domain of LTK, but only FAM150A is the specific ligand for LTK (9, 10).
- Honda, H. et al. (1999) Oncogene 18:3821.
- Yazaki, Y. et al. (1993) Proc Natl. Acad. Sci. U.S.A. 90:5404.
- Maru, Y., et al. (1990) Oncogene Res. 5:19.
- Ben-Neriah, Y. et al. (1988) Nature 333:672.
- Bernards, A. et al. (1990) EMBO J. 9:2279.
- Kozutsumi, H et al. (1994) Oncogene 9:2991.
- Yamada, S. et al. (2008) Neuroreport 19:1733.
- Roll, JD. et al. (2012) PLoS One 7:e31733.
- Reshetnyak, AV. et al. (2015) Proc Natl Acad Sci U S A. 112:15862.
- Guan, J, AV. et al. (2015) Elife. 4:e09811.
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