Recombinant Human TFF3 Protein, CF

R&D Systems | Catalog # 8294-TF

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Key Product Details

  • R&D Systems CHO-derived Recombinant Human TFF3 Protein (8294-TF)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

Q07654

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all TFF3 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human TFF3 protein
Glu22-Phe80 with a C-terminal 6-His tag

Purity

>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu22

Predicted Molecular Mass

7.4 kDa

SDS-PAGE

7-11 kDa, reducing conditions

Activity

Measured by its ability to induce ERK1/ERK2 phosphorylation in Jurkat human acute T cell leukemia cells.
5-15 μg/mL of Recombinant Human TFF3 can effectively induce ERK1/2 phosphorylation.

Formulation, Preparation, and Storage

8294-TF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TFF3

Trefoil Factor 3 (TFF3), also known as Intestinal Trefoil Factor (ITF) and P1.B, is one of three structurally related secreted proteins that contain trefoil domains. These domains adopt a three-leaved conformation held together by conserved intrachain disulfide bonds. TFF3 is an approximately 7 kDa peptide that plays an important role in epithelial regeneration and wound healing (1). It can form disulfide-linked dimers or associate into disulfide-linked complexes with the intestinal mucous proteins FCGBP and MUC-2 (2, 3). TFF3 is expressed by epithelial goblet cells in the respiratory tract, biliary and breast ducts, small and large intestine, and cardia of the stomach (4-8). Following secretion, TFF3 can be retained in the overlying mucous layer (7). TFF3 is also expressed by chondrocytes during bone development (9). Mature human TFF3 shares 76% amino acid sequence identity with mouse and rat TFF3. TFF3 is up-regulated in response to a range of gastrointestinal epithelial disruptions (8, 10). It promotes epithelial wound healing by inducing the migration of biliary, bronchial, and intestinal epithelial cells (5, 10-12). TFF3 up-regulation is associated with and enhances tumor cell invasion and metastasis (6, 13). It supports hypoxia-induced VEGF up-regulation in tumor cells and also promotes angiogenesis in non-tumor environments (14, 15). Over-expression of TFF3 in type 2 diabetic mouse liver has been shown to improve glucose tolerance and insulin sensitivity (16).

References

  1. Kjellev, S. (2009) Cell. Mol. Life Sci. 66:1350.
  2. Albert, T.K. et al. (2010) J. Proteome Res. 9:3108.
  3. Yu, H. et al. (2011) PLoS ONE 6:e20334.
  4. LeSimple, P. et al. (2007) Am. J. Respir. Cell Mol. Biol. 36:296.
  5. Nozaki, I. et al. (2004) Am. J. Pathol. 165:1907.
  6. Ahmed, A.R.H. et al. (2012) Am. J. Pathol. 180:904.
  7. Podolsky, D.K. et al. (1993) J. Biol. Chem. 268:6694.
  8. Peitz, U. et al. (2004) Peptides 25:771.
  9. Bijelic, N. et al. (2013) Acta Histochem. 115:204.
  10. Paulsen, F.P. et al. (2008) J. Biol. Chem. 283:13418.
  11. Oertel, M. et al. (2001) Am. J. Respir. Cell Mol. Biol. 25:418.
  12. Dignass, A. et al. (1994) J. Clin. Invest. 94:376.
  13. Rivat, C. et al. (2005) Cancer Res. 65:195.
  14. Rodrigues, S. et al. (2003) FASEB J. 17:7.
  15. Guleng, B. et al. (2012) Mol. Biol. Rep. 39:4127.
  16. Xue, Y. et al. (2013) PLoS ONE 8:e75240.

Long Name

Trefoil Factor 3

Alternate Names

ITF, TFI

Entrez Gene IDs

7033 (Human); 21786 (Mouse); 25563 (Rat)

Gene Symbol

TFF3

UniProt

Q07654

Additional TFF3 Products

Product Documents for Recombinant Human TFF3 Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human TFF3 Protein, CF

For research use only

Related Research Areas

Inflammatory Mediators

Citations for Recombinant Human TFF3 Protein, CF

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