Recombinant Human TMEM106B Fc Chimera Protein, CF New

Catalog #: 11644-TM Datasheet / COA / SDS

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11644-TM-050

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Recombinant Human TMEM106B Fc Chimera Protein Binding Activity.

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human TMEM106B Fc Chimera Protein, CF Summary

R&D Systems HEK293-derived Recombinant Human TMEM106B Fc Chimera Protein (11644-TM)
Quality control testing to verify active proteins with lot specific assays by in-house scientists
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Product Specifications

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. Recombinant Human TMEM106B Fc Chimera (Catalog # 11644-TM) binds Recombinant SARS-CoV-2 Spike RBD His-tag (HEK293 Expressed) (Catalog # 10500-CV) with ED₅₀ <2.00 μg/mL.

Source

Human embryonic kidney cell, HEK293-derived human TMEM106B protein

MD	Human IgG₁ (Pro100-Lys330)	IEGR	Human TREM106B (Pro118-Gln274) Accession # NP_001127704.1
N-terminus			C-terminus

N-terminal Sequence
Analysis

Met

Structure / Form

Disulfide linked homodimer

Predicted Molecular Mass

45 kDa

SDS-PAGE

>60 kDa, under reducing conditions.

Product Datasheets

11644-TM

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11644-TM

Formulation	Supplied as a 0.2 μm filtered solution in PBS with Trehalose.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after opening. 3 months, -20 to -70 °C under sterile conditions after opening.

Scientific Data

Binding Activity

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Recombinant Human TMEM106B Fc Chimera Protein (Catalog # 11644-TM) binds Recombinant SARS-CoV-2 Spike RBD His-tag (HEK293 Expressed) (10500-CV) with ED₅₀ < 2.00 μg/mL

Reconstitution Calculator

Background: TMEM106B

SARS-CoV-2 is associated with broad tissue tropism, a characteristic often determined by the availability of entry receptors on host cells. TMEM106B, a lysosomal transmembrane protein, can serve as an alternative receptor for SARS-CoV-2 entry into angiotensin-converting enzyme 2 (ACE2)-negative cells. Spike substitution E484D increased TMEM106B binding, thereby enhancing TMEM106B-mediated entry (1). ACE2-independent entry was dependent on endogenous TMEM106B, which binds to the receptor binding domain (RBD). Binding is augmented by mutation E484D (3). TMEM106B-specific monoclonal antibodies blocked SARS-CoV-2 infection, demonstrating a role of TMEM106B in viral entry. Using X-ray crystallography, cryogenic electron microscopy (cryo-EM), and hydrogen-deuterium exchange mass spectrometry (HDX-MS), shows that the luminal domain (LD) of TMEM106B engages the receptor-binding motif of SARS-CoV-2 spike (1). The large loop (RBD@471-491) could anchor TMEM106B, which was then firmly locked by another loop (RBD@444-451) (2). TMEM106B also promotes spike-mediated syncytium formation, suggesting a role of TMEM106B in viral fusion. Findings have identified an ACE2-independent SARS-CoV-2 infection mechanism that involves cooperative interactions with the receptors heparan sulfate and TMEM106B (1).

References