Recombinant Human Ubiquitin No Lys,N-Terminal Biotin, CF
Recombinant Human Ubiquitin No Lys,N-Terminal Biotin, CF Summary
Product Specifications
Lacks all reactive lysine residues (mutated to arginine) and contains a single N‑terminal biotin
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
UB-NOK
| Formulation | X mg/ml (X μM) in 10 mM HEPES pH 7.5 |
| Reconstitution | |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: Ubiquitin
Ubiquitin is a 76 amino acid (aa) protein that is ubiquitously expressed in all eukaryotic organisms. Ubiquitin is highly conserved with 96% aa sequence identity shared between human and yeast Ubiquitin, and 100% aa sequence identity shared between human and mouse Ubiquitin (1). In mammals, four Ubiquitin genes encode for two Ubiquitin-ribosomal fusion proteins and two poly-Ubiquitin proteins. Cleavage of the Ubiquitin precursors by deubiquitinating enzymes gives rise to identical Ubiquitin monomers each with a predicted molecular weight of 8.6 kDa. Conjugation of Ubiquitin to target proteins involves the formation of an isopeptide bond between the C-terminal glycine residue of Ubiquitin and a lysine residue in the target protein. This process of conjugation, referred to as ubiquitination or ubiquitylation, is a multi-step process that requires three enzymes: a Ubiquitin-activating (E1) enzyme, a Ubiquitin-conjugating (E2) enzyme, and a Ubiquitin ligase (E3). Ubiquitination is classically recognized as a mechanism to target proteins for degradation and as a result, Ubiquitin was originally named ATP-dependent Proteolysis Factor 1 (APF-1) (2,3). In addition to protein degradation, ubiquitination has been shown to mediate a variety of biological processes such as signal transduction, endocytosis, and post-endocytic sorting (4-7).
Produced via a proprietary process resulting in a single Biotin modification exclusively on the N-terminus of Ubiquitin. This site-specific modification results in a Ubiquitin that is fully functional at the C-terminus. This Ubiquitin mutant lacks all reactive lysine residues which have been mutated to arginine. These mutations render the protein unable to form poly-Ubiquitin chains and can be used as a negative control or to detect mono-ubiquitination. Detection with Avidin-linked reagents allows for a higher efficiency and detection sensitivity than with Anti-Ubiquitin antibodies. Ideal as an alternative to radio-labeled Ubiquitin.
- Sharp, P.M. & W.-H. Li. (1987) Trends Ecol. Evol. 2:328.
- Ciechanover, A. et al. (1980 ) Proc. Natl. Acad. Sci. USA 77:1365.
- Hershko, A. et al. (1980) Proc. Natl. Acad. Sci. USA 77:1783.
- Greene, W. et al. (2012) PLoS Pathog. 8:e1002703.
- Tong, X. et al. (2012) J. Biol. Chem. 287:25280.
- Wei, W. et al. (2004) Nature 428:194.
- Wertz, I.E. et al. (2004) Nature 430:694.
Citations for Recombinant Human Ubiquitin No Lys,N-Terminal Biotin, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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A ubiquitin switch controls autocatalytic inactivation of the DNA-protein crosslink repair protease SPRTN
Authors: S Zhao, A Kieser, HY Li, HK Reinking, P Weickert, S Euteneuer, D Yaneva, AC Acampora, MJ Götz, R Feederle, J Stingele
Nucleic Acids Research, 2021-01-25;0(0):.
Species: Human
Sample Types: Protein
Applications: Bioassay -
Cbl interacts with multiple E2s in vitro and in cells
Authors: MS Liyasova, K Ma, D Voeller, PE Ryan, J Chen, RE Klevit, S Lipkowitz
PLoS ONE, 2019-05-23;14(5):e0216967.
Species: Human
Sample Types: Recombinant Protein
Applications: Ubiquitination -
African swine fever virus encodes for an E2-ubiquitin conjugating enzyme that is mono- and di-ubiquitinated and required for viral replication cycle
Authors: FB Freitas, G Frouco, C Martins, F Ferreira
Sci Rep, 2018-02-22;8(1):3471.
Species: Primate
Sample Types: Recombinant Protein
Applications: Bioassay -
Development of ?-Hairpin Peptides for the Measurement of SCF-Family E3 Ligase Activity in Vitro via Ornithine Ubiquitination
Authors: KM Houston, AT Melvin, GS Woss, EL Fayer, ML Waters, NL Allbritton
ACS Omega, 2017-03-29;2(3):1198-1206.
Species: Human
Sample Types: Protein
Applications: Bioassay
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