Recombinant Mouse Axl Fc Chimera (CHO-expressed) Protein, CF

R&D Systems | Catalog # 7477-AX

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Key Product Details

  • R&D Systems CHO-derived Recombinant Mouse Axl Fc Chimera (CHO-expressed) Protein (7477-AX)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

Q00993

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all Axl Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mouse Axl protein
Mouse Axl
(His20-Pro443)
Accession # Q00993		 IEGRMDP Mouse IgG2A
(Glu98-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His20

Predicted Molecular Mass

73.6 kDa (monomer)

SDS-PAGE

90-110 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse Axl Fc Chimera is coated at 2 μg/mL (100 μL/well), the concentration of Recombinant Mouse Gas6 (Catalog # 986‑GS) that produces 50% optimal binding response is 0.4‑2 ng/mL.

Reviewed Applications

Read 1 review rated 5 using 7477-AX in the following applications:

Formulation, Preparation, and Storage

7477-AX
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Axl

Axl, also known as Ufo and Ark, is a widely expressed 140 kDa glycoprotein in the TAM receptor tyrosine kinase family. TAM family receptors (Dtk/Tyro3, Axl, and Mer) are involved in regulation of the inflammatory response, cell survival and migration, and tumorigenesis (1). Mature mouse Axl consists of a 427 aa extracellular domain (ECD) that contains two Ig-like domains and two fibronectin type III domains, a 21 aa transmembrane segment, and a 422 aa cytoplasmic domain that includes the tyrosine kinase domain (2, 3). Within the ECD, mouse Axl shares 81% and 95% aa sequence identity with human and rat Axl, respectively. Axl binds the vitamin K‑dependent protein Gas6 which triggers tyrosine autophosphorylation of the Axl cytoplasmic domain (4). Activation of Axl induces a broad range of activities including platelet aggregation and thrombus formation (5), macrophage and dendritic cell phagocytosis of apoptotic cells (6), NK cell development from hematopoietic progenitor cells (7), and in vivo angiogenesis (8). Axl is highly expressed in solid cancers and promotes in vivo tumorigenesis and tumor cell invasiveness (8, 9). It contributes to vascular remodeling and inflammatory cell infiltration in response to hypertension and restricted blood flow (10). It also functions as a cellular entry receptor for Gas6‑opsonized lentiviruses (11). A 70‑80 kDa soluble portion of the Axl ECD can be shed by proteolytic cleavage, and this fragment retains the ability to bind Gas6 (12, 13).

References

  1. Linger, R.M.A. et al. (2011) Adv. Cancer Res. 100:35.
  2. Faust, M. et al. (1992) Oncogene 7:1287.
  3. Rescigno, J. et al. (1991) Oncogene 6:1909.
  4. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
  5. Cosemans, J.M.E.M. et al. (2010) J. Thromb. Haemost. 8:1797.
  6. Seitz, H.M. et al. (2007) J. Immunol. 178:5635.
  7. Park, I.-K. et al. (2009) Blood 113:2470.
  8. Holland, S. et al. (2005) Cancer Res. 65:9294.
  9. Rankin, E.B. et al. (2010) Cancer Res. 70:7570.
  10. Korshunov, V.A. et al. (2006) Circ. Res. 98:1446.
  11. Morizono, K. et al. (2011) Cell Host Microbe 9:286.
  12. O’Bryan, J.P. et al. (1995) J. Biol. Chem. 270:551.
  13. Ekman, C. et al. (2010) J. Thromb. Haemost. 8:838.

    Long Name

    Axl Receptor Tyrosine Kinase

    Alternate Names

    AI323647, ARK, JTK11, Tyro7, UFO

    Entrez Gene IDs

    558 (Human); 26362 (Mouse); 102126361 (Cynomolgus Monkey)

    Gene Symbol

    AXL

    UniProt

    Q00993

    Additional Axl Products

    Product Documents for Recombinant Mouse Axl Fc Chimera (CHO-expressed) Protein, CF

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    Product Specific Notices for Recombinant Mouse Axl Fc Chimera (CHO-expressed) Protein, CF

    For research use only

    Citations for Recombinant Mouse Axl Fc Chimera (CHO-expressed) Protein, CF

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