Recombinant Mouse Biglycan Protein, CF Summary
- R&D Systems CHO-derived Recombinant Mouse Biglycan Protein (8128-CM)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Product Specifications
Asp38-Lys369, with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
8128-CM
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 200 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: Biglycan
Biglycan, also known as PG I, is a secreted chondroitin/dermatan sulfate proteoglycan in the small leucine-rich proteoglycan (SLRP) family. SLRP family members are characterized by N-terminal and C-terminal cysteine-rich domains that flank the central region containing 10-12 tandem leucine-rich repeats (LRRs). Biglycan function is important in the development and maintenance of many tissues (1). The mouse Biglycan cDNA encodes a 369 amino acid (aa) precursor with a 19 aa signal sequence and a 18 aa propeptide that is cleaved by BMP-1 (2). Mature mouse Biglycan shares 97% and greater than 99% aa sequence identity with human and rat Biglycan, respectively. The mature protein can be further cleaved by proteases, which compromises its inability to bind to other molecules (3, 4). The 45 kDa core protein is approximately one third the molecular weight of the fully glycanated form and can assemble into noncovalently-associated dimers (5). Biglycan binds, crosslinks, and stabilizes several proteins in the collagen matrix (6). It also binds and modulates the activity of a variety of non-matrix proteins, including C1q, collectins, TGF-beta, and Wnt3a (3, 7, 8). Biglycan binds to TLR2, TLR4, P2X4, and P2X7, leading to the increased production of inflammatory mediators, the migration of vascular endothelial cells, and the enhancement of oxidized LDL induced aortic valve calcification (9-11). Biglycan also blocks the uptake and degradation of acetylated LDL in atherosclerotic plaques by binding to SR-A (12). It stabilizes the neuromuscular junction through interactions with MuSK as well as alpha- and gamma-Sarcoglycan (13).
- Nastase, M.V. et al. (2012) J. Histochem. Cytochem. 60:963.
- Scott, I.C. et al. (2000) J. Biol. Chem. 275:30504.
- Boivin, W.A. et al. (2012) PLoS ONE 7:e33163.
- Monfort, J. et al. (2006) Arthritis Res. Ther. 8:R26.
- Scott, P.G. et al. (2006) J. Biol. Chem. 281:13324.
- Wiberg, C. et al. (2003) J. Biol. Chem. 278:37698.
- Groeneveld, T.W.L. et al. (2005) J. Immunol. 175:4715.
- Berendsen, A.D. et al. (2011) Proc. Natl. Acad. Sci. USA 108:17022.
- Babelova, A. et al. (2009) J. Biol. Chem. 284:24035.
- Yamamoto, K. et al. (2012) Br. J. Cancer 106:1214.
- Song, R. et al. (2012) Arterioscler. Thromb. Vasc. Biol. 32:2711.
- Santiago-Garcia, J. et al. (2003) J. Biol. Chem. 278:6942.
- Amenta, A.R. et al. (2012) J. Neurosci. 32:2324.
Citations for Recombinant Mouse Biglycan Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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Biglycan Alleviates Age-Related Muscle Atrophy and Hepatocellular Senescence
Authors: Lee, DS;Lim, JH;Lee, YJ;
International journal of molecular sciences
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Asthma reduces glioma formation by T cell decorin-mediated inhibition of microglia
Authors: J Chatterjee, S Sanapala, O Cobb, A Bewley, AK Goldstein, E Cordell, X Ge, JR Garbow, MJ Holtzman, DH Gutmann
Nature Communications, 2021-12-08;12(1):7122.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Adipocyte-Derived Versican and Macrophage-Derived Biglycan Control Adipose Tissue Inflammation in Obesity
Authors: CY Han, I Kang, IA Harten, JA Gebe, CK Chan, M Omer, KM Alonge, LJ den Hartig, D Gomes Kjer, L Goodspeed, S Subramania, S Wang, F Kim, DE Birk, TN Wight, A Chait
Cell Rep, 2020-06-30;31(13):107818.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
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