Recombinant Mouse BTNL6/Butyrophilin-like 6 Protein, CF

R&D Systems | Catalog # 9638-BT

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Key Product Details

  • R&D Systems CHO-derived Recombinant Mouse BTNL6/Butyrophilin-like 6 Protein (9638-BT)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

NP_109672

Applications

Bioactivity
View all BTNL6 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mouse BTNL6 protein
Lys29-Trp249, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Lys29

Predicted Molecular Mass

26 kDa

SDS-PAGE

28-38 kDa, reducing conditions

Activity

Measured by its ability to inhibit IL-2 secretion by mouse T cells in the presence of anti-CD3.
The ED50 for this effect is 0.15-0.9 μg/mL

Scientific Data Images for Recombinant Mouse BTNL6/Butyrophilin-like 6 Protein, CF

Recombinant Mouse BTNL6/Butyrophilin-like 6 Protein Bioactivity

Recombinant Mouse BTNL6/Butyrophilin-like 6 Protein Bioactivity

Recombinant Mouse BTNL6/Butyrophilin-like 6 (Catalog # 9638-BT) inhibits IL-2 secretion by mouse T cells in the presence of anti-CD3 antibody. The ED50 for this effect is 0.15-0.9 μg/mL.

Formulation, Preparation, and Storage

9638-BT
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BTNL6

Butyrophilin-like 6 (BTNL6) is a member of the BTN/MOG Ig-superfamily and functions with BTNL1 as a regulator of immune cell proliferation (1). Mouse BTNL6 is a 539 amino acid (aa) type I transmembrane glycoprotein that contains a signal peptide followed by an extracellular domain (ECD), a transmembrane region and a cytoplasmic domain. The ECD features a V-type and a C-type Ig-like domain and one glycosylation site. The Btnl6 gene is found only in mice (2). The ECD of mouse BTNL6 (aa 29-249) shares 47% and 83% sequence identity with the ECD of human BTNL2A2 and rat BTNL9, respectively. BTNL6 expression is coordinated as a heteromeric protein with BTNL1, and the presence of this complex is correlated with expansion of gamma delta T cells, especially those containing V gamma 7V delta 4 TCR (2). Btnl6 shows striking sequence similarity to Skint1; is also largely restricted to an epithelial tissue (the small intestine) replete with T cells (4). Our in-house studies showed BTNL6 co-inhibited anti-CD3 induced IL-2 secretion on CD3+ cells.

References

  1. Lebrero-Fernández C. et al. (2016) Front Immunol 7:1.
  2. Arnett, H.A. et al. (2014) Nat Rev Immunol. 14:559.
  3. Lebrero-Fernández C and Bas-Forsberg A. (2016). Sci Rep. 6:31524.
  4. Bas, A. et al. (2011) Proc. Natl Acad. Sci. USA 108:4376.

Long Name

Butyrophilin-like 6

Alternate Names

Gm6519, NG13

Entrez Gene IDs

624681 (Mouse)

Gene Symbol

BTNL6

UniProt

NP_109672

Additional BTNL6 Products

Product Documents for Recombinant Mouse BTNL6/Butyrophilin-like 6 Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse BTNL6/Butyrophilin-like 6 Protein, CF

For research use only

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