Cathepsin E is an intracellular aspartic protease of the pepsin family (1, 2). Unlike Cathepsin D, another member of the same family and a lysosomal protease with relatively ubiquitous distribution, Cathepsin E is not a lysosomal enzyme and has a limited cell and tissue distribution. However, both Cathepsins D and E play an important role in the degradation of proteins, the generation of bioactive proteins, and antigen processing (3). Both enzymes are efficient in cleaving the Swedish mutant of amyloid precursor protein (APP) at the beta site but show almost no reactivity with the wild-type APP (4). Mouse Cathepsin E is synthesized as a precursor protein, consisting of a signal peptide (residues 1‑18), a propeptide (residues 19‑59), and a mature chain (residues 60‑397) (1).
Recombinant Mouse Cathepsin E Protein, CF
R&D Systems | Catalog # 1130-AS
Key Product Details
- R&D Systems NS0-derived Recombinant Mouse Cathepsin E Protein (1130-AS)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
Accession Number
Structure / Form
Applications
Product Specifications
Source
Gln19-Pro397, with a C-terminal 10-His tag
Purity
Endotoxin Level
N-terminal Sequence Analysis
Predicted Molecular Mass
SDS-PAGE
Activity
Measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2 (Catalog # ES001).
The specific activity is >8,000 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation, and Storage
1130-AS
| Formulation | Supplied as a 0.2 μm filtered solution in MES and NaCl. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Cathepsin E
References
- Tatnell, P.J. et al. (1997) FEBS Lett. 408:62.
- Kay, J. and P.J. Tatnell (2004) in Handbook of Proteolytic Enzymes (Barrett, A.J. et al., eds.), p. 33, Academic Press, San Diego.
- Tsukuba, T. et al. (2000) Mol. Cells 10:601.
- Gruninger-Leitch, F. et al. (2000) Nat. Biotechnol. 18:66.
Alternate Names
Gene Symbol
UniProt
Additional Cathepsin E Products
Product Documents for Recombinant Mouse Cathepsin E Protein, CF
Product Specific Notices for Recombinant Mouse Cathepsin E Protein, CF
For research use only
Related Research Areas
Citations for Recombinant Mouse Cathepsin E Protein, CF
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Protocols
View specific protocols for Recombinant Mouse Cathepsin E Protein, CF (1130-AS):
- Assay Buffer: 50 mM Sodium Acetate, 100 mM NaCl, pH 3.5
- Recombinant Mouse Cathepsin E (rmCathepsin E) (Catalog # 1130-AS)
- Substrate: MCA-Pro-Leu-Gly-Leu-DPA-Ala-Arg-NH2 (Catalog # ES001)
- F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
- Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent
- Dilute rmCathepsin E to 1.0 µg/mL in Assay Buffer.
- Incubate at room temperature for 15 minutes.
- Dilute activated rmCathepsin E to 0.04 ng/µL in Assay Buffer.
- Dilute Substrate to 40 µM in Assay Buffer.
- Load 50 µL of the 0.04 ng/µL rmCathepsin E into plate and start the reaction by adding 50 µL of 40 µM Substrate. Include a Substrate Blank containing 50 µL Assay Buffer and 50 µL of 40 µM Substrate.
- Read at excitation and emission wavelengths of 320 nm and 405 nm (top read), respectively in kinetic mode for 5 minutes.
- Calculate specific activity:
|
Specific Activity (pmol/min/µg) = |
Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU) |
| amount of enzyme (µg) |
*Adjusted for Substrate Blank
**Derived using calibration standard MCA-Pro-Leu-OH (Bachem, Catalog # M-1975)
Per Well:
- rmCathepsin E: 0.002 μg
- Substrate: 20 µM