Recombinant Mouse CD155/PVR Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
9670-CD-050
Recombinant Mouse CD155/PVR Fc Chimera Protein, CF Data
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Recombinant Mouse CD155/PVR Fc Chimera Protein, CF Summary

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse CD96 (Catalog # 5690-CD) is coated at 1 μg/mL (100 μL/well), the concentration of Recombinant Mouse CD155/PVR Fc Chimera that produces 50% optimal binding response is 6-36 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse CD155/PVR protein
Mouse CD155/PVR
(Asp29-Leu348)
Accession # NP_081790
IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Asp29
Structure / Form
DIsulfide-linked homodimer
Predicted Molecular Mass
62 kDa
SDS-PAGE
92-108 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9670-CD

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity View Larger

When Recombinant Mouse CD96 (Catalog # 5690-CD) is coated at 1 µg/mL, 100 µL/well, Recombinant Mouse CD155/PVR Fc Chimera (Catalog # 9670-CD) binds with an ED50of 6-36 ng/mL.

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Reconstitution Calculator

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Background: CD155/PVR

CD155, also known as PVR (poliovirus receptor), Necl-5 (nectin-like molecule-5) and, in rodents, TAGE4 (tumor-associated glycoprotein E4), is a 70-kDa type I transmembrane glycoprotein in the nectin-related family of adhesion proteins within the immunoglobulin superfamily (1, 2). CD155, which may play a role in cancer cell invasion and migration, binds other molecules including Vitronectin, Nectin-3, DNAM-1/CD226, CD96, and TIGIT but does not bind homotypically (3, 4). Mature mouse CD155 consists of a 318 amino acid (aa) extracellular domain (ECD) with one N-terminal V-type and two C2-type Ig-like domains, a 24 aa transmembrane segment, and a 38 aa cytoplasmic tail. Within the ECD, mouse CD155 shares 46%, 73%, and 44% aa sequence identity with human CD155, rat CD155, and mouse Nectin-2, respectively. The V-type domain of CD155 mediates all binding, including to polio virus (1), and alternative splicing within this domain in humans can modulate ligand binding (5). CD155 is up-regulated on endothelial cells by IFN-gamma and is highly expressed on immature thymocytes, lymph node dendritic cells, and tumor cells of epithelial and neuronal origin (1, 2, 6-9). It is preferentially expressed on Th17 cells compared to Th2 cells (10), and its activation promotes the development of Th1 responses (11). Enhanced CD155 expression in tumor cells contributes to loss of contact inhibition and increased migration but also allows tumor cell recognition and killing by DNAM-1 or CD96 expressing NK cells (1, 7, 12). Binding of monocyte DNAM-1 to endothelial cell CD155 promotes transendothelial migration (8). The expression of CD155 on mouse CD8+ thymocytes prevents their premature exit from the thymus (13). Within intestinal Peyer's patches, follicular dendritic cell CD155 activates follicular helper T cells via DNAM-1 or CD96 binding (7-9, 14). CD155 also binds the inhibitory ligand TIGIT on NK and some mature T cells, antagonizing DNAM-1 effects (7, 14, 15).

References
  1. Mandai, K. et al. (2015) Curr. Top. Dev. Biol. 112:197.
  2. Ravens, I. et al. (2003) Biochem. Biophys. Res. Commun. 312:1364.
  3. Sloan, K. et al. (2004) BMC Cancer. 4: 73.
  4. Sato, T. et al. (2004) Genes to Cells 9:791.
  5. Meyer, D. et al. (2009) J. Biol. Chem. 284:2235.
  6. Escalante, N.K. et al. (2011) Arterioscler. Thromb. Vasc. Biol. 31:1177.
  7. Xu, Z. and B. Jin (2010) Cell. Mol. Immunol. 7:11.
  8. Reymond, N. et al. (2004) J. Exp. Med. 199:1331.
  9. Maier, M.K. et al. (2007) Eur. J. Immunol. 37 :2214.
  10. Lozano, E. et al. (2013) J. Immunol. 191:3673.
  11. Yamashita-Kanemaru, Y. et al. (2015) J. Immunol. 194:5644.
  12. Chan, C.J. et al. (2010) J. Immunol. 184:902.
  13. Qui, Q. et al. (2010) J. Immunol. 184:1681.
  14. Seth, S. et al. (2009) Eur. J. Immunol. 39:3160.
  15. Stanietsky, N. et al. (2009) Proc. Natl. Acad. Sci. USA 106:17858.
Long Name
Poliovirus Receptor
Entrez Gene IDs
5817 (Human); 52118 (Mouse); 25066 (Rat); 102136444 (Cynomolgus Monkey); 712851 (Rhesus Macaque)
Alternate Names
CD155 antigen; CD155; HVED; NECL5; Necl-5; nectin-like 5; Nectin-like protein 5; poliovirus receptor; PVR; PVS; PVSFLJ25946; Tage4

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