Recombinant Mouse CD74 Protein, CF
Recombinant Mouse CD74 Protein, CF Summary
Product Specifications
Optimal dilutions should be determined by each laboratory for each application.
Gln56-Leu215, with an N-terminal HA-tag (YPYDVPDYA)
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
7478-CD
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: CD74
CD74, also known as Invariant chain (Ii) and p33, is a type 2 transmembrane glycoprotein that plays an important role in adaptive immunity, inflammation, and cancer (1). Mature mouse CD74 consists of a 29 amino acid (aa) cytoplasmic domain, a 29 aa transmembrane segment, and a 224 aa extracellular domain (ECD) that contains one thyroglobulin type 1 domain (2). Alternate splicing generates a short isoform that lacks the thyroglobulin domain (2). Within the ECD, mouse CD74 shares 75% and 88% aa sequence identity with human and rat CD74, respectively. CD74 functions as a chaperone for MHC class II molecules on antigen presenting cells and undergoes progressive proteolysis during class II trafficking and antigenic peptide loading (3). Full length CD74 assembles into trimers which then associate with class II molecules in nonameric complexes on the cell surface (4, 5). CD74 also associates with CD44 and binds with high affinity to the cytokine MIF, leading to inflammatory leukocyte responses, protection from tissue fibrosis, B cell proliferative and survival signaling, and the up‑regulation of angiogenic factors in endometrial stromal cells (6‑11). MIF binding notably induces the proteolytic cleavage of the CD74 intracellular domain which then promotes B cell differentiation (10). CD74 is up‑regulated on non‑immune cells at sites of inflammation including amyloid beta plaques and atherosclerotic plaques (12, 13). It is also up‑regulated in a variety of cancers and enhances tumorigenicity, tumor angiogenesis, and metastasis (1, 14).
- Beswick, E.J. and V.E. Reyes (2009) World J. Gastroenterol. 15:2855.
- Koch, N. et al. (1987) EMBO J. 6:1677.
- Riberdy, J.M. et al. (1992) Nature 360:474.
- Koch, N. et al. (1991) J. Immunol. 147:2643.
- Roche, P.A. et al. (1991) Nature 354:392.
- Leng, L. et al. (2003) J. Exp. Med. 197:1467.
- Takahashi, K. et al. (2009) Respir. Res. 10:33.
- Heinrichs, D. et al. (2011) Proc. Natl. Acad. Sci. 104:17444.
- Shi, X. et al. (2006) Immunity 25:595.
- Gore, Y. et al. (2008) J. Biol. Chem. 283:2784.
- Veillat, V. et al. (2010) J. Clin. Endocrinol. Metab. 95:E403.
- Bryan, K.J. et al. (2008) Mol. Neurodegen. 3:13.
- Martin-Ventura, J.L. et al. (2009) Cardiovasc. Res. 83:586.
- Liu, Y.-H. et al. (2008) J. Immunol. 181:6584.
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