Recombinant Mouse Contactin-6 Protein, CF

Catalog # Availability Size / Price Qty
5890-CN-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Mouse Contactin-6 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of Neuro‑2A mouse neuroblastoma cells.

When 5 x 104 cells/well are added to rmContactin-6 coated plates (10 µg/mL, 100 µL/well), approximately 40‑60% will adhere after 30 minutes at 37 °C.

Optimal dilutions should be determined by each laboratory for each application.

Source
Chinese Hamster Ovary cell line, CHO-derived mouse Contactin-6 protein
Glu20-Ser998, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Glu20
Predicted Molecular Mass
109.2 kDa
SDS-PAGE
140-160 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5890-CN

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Contactin-6

Contactin-6, also known as NB-3, is a 130 kDa member of the TAG/F3 subgroup of Contactin GPI-linked neuronal adhesion proteins (1, 2). Mature mouse Contactin-6 consists of six immunoglobulin-like domains, a flexible linker region, four fibronectin type III domains, and a GPI anchor (3 - 5). Mouse Contactin-6 shares 90% and 96% amino acid (aa) sequence identity with human and rat Contactin-6, respectively. Alternate splicing generates an isoform with a 17 aa deletion in the first Ig-like domain (3). Contactin-6 expression is upregulated postnatally in the cerebellum, brainstem, and hippocampus, and it shows differential expression within various structures of the brain (3, 5 - 7). In the cerebellum, it is presynaptically localized at synapses between excitatory glutamatergic parallel fibers and Purkinje cells (7). Contactin-6 associates in cis with CHL-1 and in trans with Notch-1 on oligodendrocytes (6, 8). Its binding to Notch-1 triggers translocation of the Notch intracellular domain to the nucleus and promotes oligodendrogenesis (6). Contactin-6 function is important in the postnatal development and function of the cerebellum, as shown in knockout mice which exhibit poor motor coordination (5, 7).

References
  1. Shimoda, Y. and K. Watanabe (2009) Cell Adhesion Migration 3:64.
  2. Hu, Q.D. et al. (2006) Dev. Neurosci. 28:25.
  3. Lee, S. et al. (2000) Gene 245:253.
  4. Ogawa, J. et al. (1996) Neurosci. Lett. 218:173.
  5. Takeda, Y. et al. (2003) J. Neurobiol. 56:252.
  6. Cui, X.-Y. et al. (2004) J. Biol. Chem. 279:25858.
  7. Sakurai, K. et al. (2009) Dev. Neurobiol. Aug 11 epub.
  8. Ye, H. et al. (2008) EMBO J. 27:188.
Entrez Gene IDs
27255 (Human); 53870 (Mouse); 27256 (Rat)
Alternate Names
CNTN6; contactin 6; Contactin6; Contactin-6; MGC133256; NB3

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