Recombinant Mouse Contactin-6 Protein, CF Summary
When 5 x 104 cells/well are added to rmContactin-6 coated plates (10 µg/mL, 100 µL/well), approximately 40‑60% will adhere after 30 minutes at 37 °C.
Optimal dilutions should be determined by each laboratory for each application.
Glu20-Ser998, with a C-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 100 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Contactin-6, also known as NB-3, is a 130 kDa member of the TAG/F3 subgroup of Contactin GPI-linked neuronal adhesion proteins (1, 2). Mature mouse Contactin-6 consists of six immunoglobulin-like domains, a flexible linker region, four fibronectin type III domains, and a GPI anchor (3 - 5). Mouse Contactin-6 shares 90% and 96% amino acid (aa) sequence identity with human and rat Contactin-6, respectively. Alternate splicing generates an isoform with a 17 aa deletion in the first Ig-like domain (3). Contactin-6 expression is upregulated postnatally in the cerebellum, brainstem, and hippocampus, and it shows differential expression within various structures of the brain (3, 5 - 7). In the cerebellum, it is presynaptically localized at synapses between excitatory glutamatergic parallel fibers and Purkinje cells (7). Contactin-6 associates in cis with CHL-1 and in trans with Notch-1 on oligodendrocytes (6, 8). Its binding to Notch-1 triggers translocation of the Notch intracellular domain to the nucleus and promotes oligodendrogenesis (6). Contactin-6 function is important in the postnatal development and function of the cerebellum, as shown in knockout mice which exhibit poor motor coordination (5, 7).
- Shimoda, Y. and K. Watanabe (2009) Cell Adhesion Migration 3:64.
- Hu, Q.D. et al. (2006) Dev. Neurosci. 28:25.
- Lee, S. et al. (2000) Gene 245:253.
- Ogawa, J. et al. (1996) Neurosci. Lett. 218:173.
- Takeda, Y. et al. (2003) J. Neurobiol. 56:252.
- Cui, X.-Y. et al. (2004) J. Biol. Chem. 279:25858.
- Sakurai, K. et al. (2009) Dev. Neurobiol. Aug 11 epub.
- Ye, H. et al. (2008) EMBO J. 27:188.
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