Recombinant Mouse CRELD2 Protein, CF

R&D Systems | Catalog # 3686-CR

R&D Systems
100% Guarantee

Key Product Details

  • R&D Systems NS0-derived Recombinant Mouse CRELD2 Protein (3686-CR)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

Q9CYA0

Applications

Bioactivity
View all CRELD2 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse CRELD2 protein
Ser23-Leu350, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser23

Predicted Molecular Mass

36.8 kDa

SDS-PAGE

57-62 kDa, reducing conditions

Activity

Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells.
The ED50 for this effect is typically 0.75-3 μg/mL.

Formulation, Preparation, and Storage

3686-CR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: CRELD2

Cysteine‑rich with EGF‑like domain protein 2 (CRELD2) is an approximately 60 kDa glycoprotein that contains two EGF‑like domains and two FU domains (1). Mature mouse CRELD2 shares approximately 77% and 92% aa sequence identity with human and rat CRELD2, respectively. It is widely expressed in fetal and adult tissues (1, 2). CRELD2 localizes to the endoplasmic reticulum and Golgi and can also be secreted (2‑4). It is up‑regulated during the cellular stress‑induced unfolded protein response (UPR), cartilage and bone pathologies (MED and MCDS), and arsenic‑induced liver toxicity (3, 5‑7). CRELD2 interacts intracellularly with the alpha 4 and beta 2 subunits of the nicotinic acetylcholine receptor and inhibits cell surface expression of the receptor (2, 8). CRELD2 expression is up‑regulated in prostate cancer by dihydroxytestosterone and is down‑regulated following anti‑androgen treatment (9).

References

  1. Rupp, P.A. et al. (2002) Gene 293:47.
  2. Ortiz, J.A. et al. (2005) J. Neurochem. 95:1585.
  3. Oh-hashi, K. et al. (2009) Biochem. Biophys. Res. Commun. 387:504.
  4. Oh-hashi, K. et al. (2011) FEBS Lett. 585:2481.
  5. Cameron, T.L. et al. (2011) PLoS ONE 6:e24600.
  6. Nundlall, S. et al. (2010) Cell Stress Chaperones 15:835.
  7. Nohara, K. et al. (2012) Toxicol. Sci. epub.
  8. Hosur, V. et al. (2009) J. Neurochem. 111:848.
  9. Jariwala, U. et al. (2007) Mol. Cancer 6:39.

Long Name

Cysteine-rich Protein With EGF-like Domains 2

Alternate Names

cysteine-rich with EGF-like domain protein 2, cysteine-rich with EGF-like domains 2, DKFZp667O055, MGC11256

Entrez Gene IDs

79174 (Human); 76737 (Mouse)

Gene Symbol

CRELD2

UniProt

Q9CYA0 (Mouse)

Additional CRELD2 Products

Product Documents for Recombinant Mouse CRELD2 Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Mouse CRELD2 Protein, CF

For research use only

Citations for Recombinant Mouse CRELD2 Protein, CF

Customer Reviews for Recombinant Mouse CRELD2 Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Mouse CRELD2 Protein, CF and earn rewards!

Have you used Recombinant Mouse CRELD2 Protein, CF?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Proteins and Enzymes