Recombinant Mouse CXCL2/MIP-2 Protein

R&D Systems | Catalog # 452-M2

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Key Product Details

  • R&D Systems E. coli-derived Recombinant Mouse CXCL2/MIP-2 Protein (452-M2)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Accession Number

P10889

Applications

Bioactivity
View all CXCL2/GRO beta/MIP-2/CINC-3 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived mouse CXCL2/GRO beta/MIP-2/CINC-3 protein
Ala28-Asn100

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala28

Predicted Molecular Mass

8 kDa

Activity

Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CXCR2.
The ED50 for this effect is 0.500‑5.00 ng/mL.

Reviewed Applications

Read 4 reviews rated 4.5 using 452-M2 in the following applications:

Formulation, Preparation, and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 452-M2
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 452-M2/CF
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CXCL2/GRO beta/MIP-2/CINC-3

Macrophage Inflammatory Protein-2 (MIP-2) was originally identified as a heparin-binding protein secreted from a murine macrophage cell line in response to endotoxin stimulation. Based on its protein and DNA sequences, MIP-2 is a member of the alpha (C-X-C) subfamily of chemokines.

MIP-2 cDNA encodes a 100 amino acid residue precursor protein from which the amino-terminal 27 amino acid residues are cleaved to generate the mature MIP-2. The protein sequence of murine MIP-2 shows approximately 63% identity to that of murine KC, another murine alpha chemokine whose expression is induced by PDGF. In addition, the protein sequence of MIP-2 is also 60% identical to human GRO beta and GRO gamma. It has been suggested that mouse KC and MIP-2 are the homologs of the human GROs and rat CINCs.

Similarly to other alpha chemokines, murine MIP-2 is a potent neutrophil attractant and activator. MIP-2 and KC can bind the murine interleukin 8 type B receptor homologue with high affinity. The expression of MIP-2 was found to be associated with neutrophil influx in pulmonary inflammation and glomerulonephritis, suggesting that MIP-2 may contribute to the pathogenesis of inflammatory diseases.

Alternate Names

CINC-3, CINC3, GRO beta, MGSA-beta, MIP-2

Entrez Gene IDs

2920 (Human); 20310 (Mouse); 114105 (Rat)

Gene Symbol

CXCL2

UniProt

P10889

Additional CXCL2/GRO beta/MIP-2/CINC-3 Products

Product Documents for Recombinant Mouse CXCL2/MIP-2 Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 452-M2 (with carrier)
Download SDS 452-M2/CF (carrier free)

Product Specific Notices for Recombinant Mouse CXCL2/MIP-2 Protein

For research use only

Citations for Recombinant Mouse CXCL2/MIP-2 Protein

Customer Reviews for Recombinant Mouse CXCL2/MIP-2 Protein (4)

4.5 out of 5
4 Customer Ratings
5 Stars
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4 Stars
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3 Stars
0%
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1 Stars
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  • Recombinant Mouse CXCL2/MIP-2 Protein
    Name: Anonymous
    Application: Cell migration/motility
    Verified Customer | Posted 11/13/2019
  • Recombinant Mouse CXCL2/MIP-2 Protein
    Name: Jim Hsiao
    Application: Cell migration/motility
    Verified Customer | Posted 04/17/2018
    A total of 10^6 mouse neutrophils was added to an insert of a transwell. Recombinant mouse MIP-2 (100 ng/ml) was used as a neutrophil chemoattractant and was added to the lower chamber. After 2 hours of incubation, migrated neutrophils in the lower chambers were enumerated.
    Recombinant Mouse CXCL2/MIP-2 Protein 452-M2
  • Recombinant Mouse CXCL2/MIP-2 Protein
    Name: James Xiao
    Application: Cell migration/motility
    Verified Customer | Posted 04/17/2018
    A total of 10^6 mouse neutrophils was added to an insert of a transwell. Recombinant mouse MIP-2 (100 ng/ml) was used as a neutrophil chemoattractant and was added to the lower chamber. After 2 hours of incubation, migrated neutrophils in the lower chambers were enumerated.
    Recombinant Mouse CXCL2/MIP-2 Protein 452-M2
  • Recombinant Mouse CXCL2/MIP-2 Protein
    Name: Anonymous
    Application: Immunoassay Standard
    Verified Customer | Posted 01/05/2018
    We made a sandwich ELISA for Mouse MIP-2. MAB452 was used as the capture. BAF452 was used as the detection. 452-M2 was used as the immunoassay standard. It worked well.
    Recombinant Mouse CXCL2/MIP-2 Protein 452-M2

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