Recombinant Mouse DLL1 Fc Chimera Protein, CF

R&D Systems | Catalog # 5026-DL

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Key Product Details

  • R&D Systems NS0-derived Recombinant Mouse DLL1 Fc Chimera Protein (5026-DL)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

Q61483

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all DLL1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse DLL1 protein
Mouse DLL1
(Ser22-Gln516)
Accession # Q61483		 LIEGRMDP Mouse IgG2A
(Glu98-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser22

Predicted Molecular Mass

81.4 kDa (monomer)

SDS-PAGE

94 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. Nobta, M. et al. (2005) J. Biol. Chem. 280:15842.
The ED50 for this effect is 0.25-1 μg/mL.

Reviewed Applications

Read 2 reviews rated 4.5 using 5026-DL in the following applications:

Formulation, Preparation, and Storage

5026-DL
Formulation Lyophilized from a 0.2 μm filtered solution in HEPES and EDTA.
Reconstitution

Reconstitute at 500 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: DLL1

Delta-like protein 1 (DLL1) is a 90-100 kDa type I transmembrane protein in the Delta/Serrate/Lag-2 (DSL) family of Notch ligands. Mature mouse DLL1 consists of a 528 amino acid (aa) extracellular domain (ECD) with one DSL domain and eight EGF-like repeats, a 23 aa transmembrane segment, and a 154 aa cytoplasmic domain (1). Within the ECD, mouse DLL1 shares 91% and 95% aa sequence identity with human and rat DLL1, respectively. It shares 26%, 35%, and 51% aa sequence identity with DLL2, 3, and 4, respectively. A 60 kDa ECD fragment, released by ADAM9, 12, or 17 mediated proteolysis, promotes the proliferation of hematopoietic progenitor cells (2, 3). The residual membrane-bound portion of DLL1 can be cleaved by presenilin-dependent gamma -secretase, enabling the cytoplasmic domain to migrate to the nucleus (4). DLL1 localizes to adherens junctions on neuronal processes through its association with the scaffolding protein MAGI1 (5). DLL1 is widely expressed, and it plays an important role in embryonic somite formation, cochlear hair cell differentiation, lymphocyte differentiation, and the maintenance of neural and myogenic progenitor cells (6-12). The up-regulation of DLL1 in arterial endothelial cells following injury or angiogenic stimulation is central to postnatal arteriogenesis (13). DLL1 is also over-expressed in cervical carcinoma and glioma and contributes to tumor progression (14, 15).

References

  1. Bettenhausen, B. et al. (1995) Development 121:2407.
  2. Dyczynska, E. et al. (2007) J. Biol. Chem. 282:436.
  3. Karanu, F.N. et al. (2001) Blood 97:1960.
  4. Ikeuchi, T. and S.S. Sisodia (2003) J. Biol. Chem. 278:7751.
  5. Mizuhara, E. et al. (2005) J. Biol. Chem. 280:26499.
  6. Takahashi, Y. et al. (2003) Development 130:4259.
  7. Teppner, I. et al. (2007) BMC Dev. Biol. 7:68.
  8. Kiernan, A.E. et al. (2005) Development 132:4353.
  9. Schmitt, T.M. and J.C. Zuniga-Pflucker (2002) Immunity 17:749.
  10. Hozumi, K. et al. (2004) Nat. Immunol. 5:638.
  11. Shimojo, H. et al. (2008) Neuron 58:52.
  12. Schuster-Gossler, K. et al. (2007) Proc. Natl. Acad. Sci. 104:537.
  13. Limbourg, A. et al. (2007) Circ. Res. 100:363.
  14. Purow, B.W. et al. (2005) Cancer Res. 65:2353.
  15. Gray, G.E. et al. (1999) Am. J. Pathol. 154:785.

Long Name

Delta-like 1

Alternate Names

Delta 1

Entrez Gene IDs

28514 (Human); 13388 (Mouse); 84010 (Rat)

Gene Symbol

DLL1

UniProt

Q61483

Additional DLL1 Products

Product Documents for Recombinant Mouse DLL1 Fc Chimera Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse DLL1 Fc Chimera Protein, CF

For research use only

Related Research Areas

Notch Pathway
Somite Markers

Citations for Recombinant Mouse DLL1 Fc Chimera Protein, CF

Customer Reviews for Recombinant Mouse DLL1 Fc Chimera Protein, CF (2)

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  • Recombinant Mouse DLL1 Fc Chimera Protein, CF
    Name: Anonymous
    Application: Cell Proliferation
    Verified Customer | Posted 10/13/2018
  • Recombinant Mouse DLL1 Fc Chimera Protein, CF
    Name: Pawel Mazur
    Application: Cell Proliferation
    Verified Customer | Posted 10/21/2016
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Associated Pathways

Notch Signaling Pathways Notch Signaling Pathway Thumbnail