Recombinant Mouse EphA5 Protein, CF
Recombinant Mouse EphA5 Protein, CF Summary
Ser58-Pro412, with a C-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 100 μg/mL in sterile PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
EphA5, also known as Ehk1, Bsk, Cek7, Hek7, and Rek7, is a member of the Eph receptor tyrosine kinase family which binds members of the Ephrin ligand family (1 - 4). The A and B class Eph proteins have a common structural organization. Activation of kinase activity occurs after membrane-bound or clustered ligand recognition and binding. Reverse signaling is propagated through the Ephrin ligand. The mouse EphA5 cDNA encodes an 877 amino acid (aa) precursor that includes a 26 aa signal sequence, a 386 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 444 aa cytoplasmic domain (5). The ECD contains an N-terminal globular domain, a cysteine-rich domain, and two fibronectin type III domains. The cytoplasmic domain contains a juxtamembrane motif with two tyrosine residues, which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) (5). Taking into account differences in splice variants between species, comparable regions of the mouse EphA5 ECD share greater than 97% aa sequence identity with bovine, human, and rat EphA5, and 27 - 43% aa identity with mouse EphA1, 2, 3, 4, 6, 7, and 8. Multiple EphA5 splice variants exist and differ in their extracellular and intracellular sequences (5). Mouse EphA5 is expressed exclusively in brain with the highest levels in the hippocampus and limbic system (5). During embryonal development, EphA5 is preferentially expressed in neurons migrating away from the ventricular zone (5). The interaction of EphA5 with its ligands is also involved in the topographic projection of hippocampal, olfactory, and retinotectal neurons (6). Interference in this system by the transgenic expression of soluble EphA5 implicates hippocampal post-synaptic areas for native EphA5 function (7).
- Poliakov, A. et al. (2004) Dev. Cell 7:465.
- Surawska, H. et al. (2004) Cytokine Growth Factor Rev. 15:419.
- Pasquale, E.B. (2005) Nat. Rev. Mol. Cell Biol. 6:462.
- Davy, A. and P. Soriano (2005) Dev. Dyn. 232:1.
- Zhou, R. et al. (1994) J. Neurosci. Res. 37:129.
- Zhou, R. (1997) Cell Tissue Res. 290:251.
- Martinez, A. et al. (2005) Neuroscience 135:451.
Citations for Recombinant Mouse EphA5 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 3
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SpiCee: A Genetic Tool for Subcellular and Cell-Specific Calcium Manipulation
Authors: O Ros, S Baudet, Y Zagar, K Loulier, F Roche, S Couvet, A Aghaie, M Atkins, A Louail, C Petit, C Metin, Y Mechulam, X Nicol
Cell Rep, 2020-07-21;32(3):107934.
Sample Types: Whole Tissue
Applications: Cell Culture
Primary cilia control glucose homeostasis via islet paracrine interactions
Authors: JW Hughes, JH Cho, HE Conway, MR DiGruccio, XW Ng, HF Roseman, D Abreu, F Urano, DW Piston
Proc. Natl. Acad. Sci. U.S.A., 2020-04-06;117(16):8912-8923.
Sample Types: Whole Tissue
EphA-Ephrin-A-mediated beta cell communication regulates insulin secretion from pancreatic islets.
Authors: Konstantinova I, Nikolova G, Ohara-Imaizumi M, Meda P, Kucera T, Zarbalis K, Wurst W, Nagamatsu S, Lammert E
Sample Types: Whole Cells, Whole Tissue
Applications: Bioassay, IHC
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