Recombinant Mouse FCRLB/FCRY Protein, CF

R&D Systems | Catalog # 4868-FC

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Key Product Details

  • R&D Systems NS0-derived Recombinant Mouse FCRLB/FCRY Protein (4868-FC)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

Q5DRQ8

Applications

Bioactivity
View all FCRLB/FCRY Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse FCRLB/FCRY protein
Ala18-Ser427, with a C-terminal 6-His tag

Purity

>80%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala18

Predicted Molecular Mass

46.5 kDa (monomer)

SDS-PAGE

60-70 kDa, reducing conditions

Activity

Measured by its ability to bind mouse IgG with an estimated KD < 300 nM.

Formulation, Preparation, and Storage

4868-FC
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FCRLB/FCRY

FCRLB, also known as FCRL2, FCRY, and FREB2, is a 60 kDa protein with sequence homology to classical Fc receptors. There are at least six type 1 transmembrane FCRL proteins and two that lack transmembrane segments. Each family member contains between three and nine immunoglobulin-like domains. FCRL proteins are differentially expressed within the B cell lineage and can either promote or inhibit B cell proliferation and activation (1, 2). According to R&D Systems testing, FCRLB binds to purified mouse IgG. Mouse FCRLB shares 82% and 94% aa sequence identity with human and rat FCRLB, respectively. It contains a putative signal peptide, three immunoglobulin-like domains, and a mucin-like stalk that is rich in Pro, Ser, and Thr residues (1 - 3). When expressed in transfectants, FCRLB is not secreted but shows a diffuse intracellular localization (4, 5). FCRLB is expressed at a low level in placenta and in B lineage cells of the germinal center (4, 5). It is up‑regulated in B cells by BAFF and LPS (3). Among non‑hematopoietic cells, FCRLB is expressed in fibroblasts, melanocytes, and melanoma (3, 4, 6). It is preferentially expressed in non‑proliferating cells and at the onset of apoptosis (3, 5).

References

  1. Davis, R.S. (2007) Annu. Rev. Immunol. 25:525.
  2. Maltais, L.J. et al. (2006) Nat. Immunol. 7:431.
  3. Masuda, K. et al. (2005) Gene 363:32.
  4. Chikaev, N.A. et al. (2005) Genomics 85:264.
  5. Wilson, T.J. and M. Colonna (2005) Genes Immun. 6:341.
  6. Inozume, T. et al. (2005) Int. J. Cancer 114:283.

Long Name

Fc Receptor-like B

Alternate Names

FcRL2, FCRLM2, FCRLY, FCRY, FREB-2

Entrez Gene IDs

127943 (Human); 435653 (Mouse); 498275 (Rat)

Gene Symbol

FCRLB

UniProt

Q5DRQ8

Additional FCRLB/FCRY Products

Product Documents for Recombinant Mouse FCRLB/FCRY Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse FCRLB/FCRY Protein, CF

For research use only

Related Research Areas

B Cell Activation
Fc Receptors

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