Recombinant Mouse IL-17A Protein

R&D Systems | Catalog # 421-ML

New Version Available: 7956-ML/CF
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Key Product Details

  • R&D Systems E. coli-derived Recombinant Mouse IL-17A Protein (421-ML)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Accession Number

Q62386.1

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all IL-17/IL-17A Proteins and Enzymes »

Product Specifications

Source

E. coli-derived mouse IL-17/IL-17A protein
Thr22-Ala158

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Thr22

Predicted Molecular Mass

15.5 kDa (monomer)

Activity

Measured by its ability to induce IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells. Yao, Z. et al. (1995) Immunity 3:811.
The ED50 for this effect is 0.25-1.25 ng/mL.

Reviewed Applications

Read 7 reviews rated 4.6 using 421-ML in the following applications:

Formulation, Preparation, and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 421-ML
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 25 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 421-ML/CF
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-17/IL-17A

Interleukin-17A (IL-17A), also known as CTLA-8, is a 15-20 kDa glycosylated cytokine that plays an important role in anti-microbial and chronic inflammation. The six IL-17 cytokines (IL-17A-F) are encoded by separate genes but adopt a conserved cystine knot fold (1, 2). Mature mouse IL-17A shares 61% and 89% amino acid sequence identity with human and rat IL-17A, respectively (3, 4). IL-17A is secreted by Th17 cells, gamma / delta T cells, iNKT cells, NK cells, LTi cells, neutrophils, and intestinal Paneth cells (2). It forms disulfide-linked homodimers as well as disulfide-linked heterodimers with IL-17F (5, 6). IL-17A exerts its effects through the transmembrane IL-17RA in complex with IL-17RC or IL-17RD (7, 8). Both IL-17RA and IL-17RC are required for responsiveness to heterodimeric IL-17A/F (7). IL-17A promotes protective mucosal and epidermal inflammation in response to microbial infection (9-12). It induces chemokine production, neutrophil influx, and the production of antibacterial peptides (9-11). IL-17A/F likewise induces neutrophil migration, but IL-17F does not (11). IL-17A additionally enhances the production of inflammatory mediators by rheumatoid synovial fibroblasts and contributes to TNF-alpha induced shock (Fossiez, 14). In contrast, it can protect against the progression of colitis by limiting chronic inflammation (12). IL-17A encourages the formation of autoreactive germinal centers and exacerbates the onset and progression of experimental models of autoimmunity (15, 16). IL‑17A has been shown to exert either tumorigenic or anti-tumor effects (17, 18).

References

  1. Gaffen, S.L. (2009) Nat. Rev. Immunol. 9:556.
  2. Cua, D.J. and C.M. Tato (2010) Nat. Rev. Immunol. 10:479.
  3. Yao, Z. et al. (1996) Gene 168:223.
  4. Rouvier, E. et al. (1993) J. Immunol. 150:5445.
  5. Chang, S.H. and C. Dong (2007) Cell Res. 17:435.
  6. Wright, J.F. et al. (2007) J. Biol. Chem. 282:13447.
  7. Wright, J.F. et al. (2008) J. Immunol. 181:2799.
  8. Rong, Z. et al. (2009) Cell Res. 19:208.
  9. Cho, J.S. et al. (2010) J. Clin. Invest. 120:1762.
  10. Liang, S.C. et al. (2006) J. Exp. Med. 203:2271.
  11. Liang, S.C. et al. (2007) J. Immunol. 179:7791.
  12. O’Connor Jr., W. et al. (2009) Nat. Immunol. 10:603.
  13. Fossiez, F. et al. (1996) J. Exp. Med. 183:2593.
  14. Takahashi, N. et al. (2008) J. Exp. Med. 205:1755.
  15. Hsu, H. et al. (2008) Nat. Immunol. 9:166.
  16. Rohn, T.A. et al. (2006) Eur. J. Immunol. 36:2857.
  17. Wang, L. et al. (2009) J. Exp. Med. 206:1457.
  18. Kryczek, I. et al. (2009) Blood 114:357.

Long Name

Interleukin 17

Alternate Names

CTLA-8, CTLA8, IL-17A, IL17, IL17A

Entrez Gene IDs

3605 (Human); 16171 (Mouse); 301289 (Rat); 449530 (Porcine); 481837 (Canine); 102119976 (Cynomolgus Monkey)

Gene Symbol

IL17A

UniProt

Q62386.1

Additional IL-17/IL-17A Products

Product Documents for Recombinant Mouse IL-17A Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 421-ML (with carrier)
Download SDS 421-ML/CF (carrier free)

Product Specific Notices for Recombinant Mouse IL-17A Protein

For research use only

Citations for Recombinant Mouse IL-17A Protein

Customer Reviews for Recombinant Mouse IL-17A Protein (7)

4.6 out of 5
7 Customer Ratings
5 Stars
71%
4 Stars
14%
3 Stars
14%
2 Stars
0%
1 Stars
0%

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  • Recombinant Mouse IL-17A Protein
    Name: Antia Veal
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 02/20/2019
  • Recombinant Mouse IL-17A Protein
    Name: Anonymous
    Application: Characterization prior to use.
    Verified Customer | Posted 02/12/2018
    Recombinant Mouse IL-17A Protein 421-ML
  • Recombinant Mouse IL-17A Protein
    Name: Maryjane DeBusk
    Application: In vivo study
    Verified Customer | Posted 12/01/2017
  • Recombinant Mouse IL-17A Protein
    Name: Anonymous
    Application: Immunoassay Standard
    Verified Customer | Posted 11/27/2017
    This protein was used as the calibrator in an ELISA using antibodies MAB421 and BAF421 as the matched pair.
    Recombinant Mouse IL-17A Protein 421-ML
  • Name: Boubacar Barry
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 06/01/2017
  • Recombinant Mouse IL-17A Protein
    Name: Yuyan Cheng
    Application: Immunoassay Standard
    Verified Customer | Posted 05/20/2016
    Recombinant Mouse IL-17A Protein 421-ML
  • Recombinant Mouse IL-17A Protein
    Name: Anonymous
    Application: injection to mice
    Verified Customer | Posted 05/16/2016

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