Limitin is a secreted interferon (IFN)-like glycoprotein. It has antiviral activity and selectively inhibits B-lineage lymphoid-cell development both in vivo and in vitro, while having little effect on myelopoiesis or erythropoiesis. Mouse Limitin cDNA encodes a 182 amino acid (aa) residue precursor protein with an N-terminal 21 aa residue putative signal peptide. Limitin binds to the IFN-alpha / beta receptors and induces IFN regulatory factor-1. Mouse Limitin displays 32%, 26% and 30% amino acid identity with mouse IFN-alpha, mouse IFN-beta, and human IFN-omega, respectively.
Recombinant Mouse Limitin/IFN-zeta Fc Chimera Protein, CF
R&D Systems | Catalog # 597-LM
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Key Product Details
- R&D Systems NS0-derived Recombinant Mouse Limitin/IFN-zeta Fc Chimera Protein (597-LM)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
NS0
Accession Number
Structure / Form
Disulfide-linked homodimer
Applications
Bioactivity
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Product Specifications
Source
Mouse myeloma cell line, NS0-derived mouse Limitin/IFN-zeta protein
| Mouse Limitin (Leu22 - Arg182) Accession # BAA83749 |
IEGRMD | Human IgG1 (Pro100 - Lys330) |
| N-terminus | C-terminus |
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Leu22
Predicted Molecular Mass
45.0 kDa (monomer)
SDS-PAGE
50-55 kDa, reducing conditions
Activity
Measured in an anti-viral assay using L‑929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. Vogel, S.N. et al. (1982) Infect. Immunol. 38:681.
The ED50 for this effect is 0.1-0.5 ng/mL.
The ED50 for this effect is 0.1-0.5 ng/mL.
Formulation, Preparation, and Storage
597-LM
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS.
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| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Calculators
Background: Limitin/IFN-zeta
References
- Oritani, K. et al. (2001) J. Mol. Med. 79:168.
- Oritani, K. et al. (2000) Nature Med. 6:659.
Long Name
Interferon zeta
Alternate Names
IFN-z, IFNzeta
Entrez Gene IDs
319146 (Mouse)
Gene Symbol
IFNZ
UniProt
Additional Limitin/IFN-zeta Products
Product Documents for Recombinant Mouse Limitin/IFN-zeta Fc Chimera Protein, CF
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Mouse Limitin/IFN-zeta Fc Chimera Protein, CF
For research use only
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Associated Pathways
