Recombinant Mouse Semaphorin 5B Fc Chimera Protein, CF

R&D Systems | Catalog # 6766-S5

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Key Product Details

  • R&D Systems CHO-derived Recombinant Mouse Semaphorin 5B Fc Chimera Protein (6766-S5)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

AAH52397

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all Semaphorin 5B Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mouse Semaphorin 5B protein
Mouse Semaphorin 5B
(Met1 - Thr805)
Accession # AAH52397		 IEGRMDP Mouse IgG2A
(Glu98 - Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained, sequencing might be blocked: Gln27 is predicted

Predicted Molecular Mass

113.3 kDa (monomer)

SDS-PAGE

130-150 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of human pancreatic cancer cells.
When 5 x 104 cells/well are added to Recombinant Mouse Semaphorin 5B Fc Chimera coated plates, cell adhesion is enhanced in a dose dependent manner after 1 hour at 37 °C.  The ED50 for this effect is 1.0-4.0 μg/mL.

Formulation, Preparation, and Storage

6766-S5
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Semaphorin 5B

Semaphorin 5B (Sema5B, previously called SemaG or SemG) is a ~150 kDa protein of the semaphorin family of axon guidance molecules (1, 2). Sema5B is a Class 5 semaphorin and this makes it a single‑pass transmembrane glycoprotein with an N‑terminal Sema domain and multiple juxtamembrane type 1 thrombospondin (TSP) repeats within its extracellular region (1, 2). Sema5B is expressed developmentally in the neuroepithelium along the anterior‑posterior axis in a complimentary pattern with Sema5A (2, 3). It has been identified in oligodendrocytes and their precursors, neurons, dental mesenchyme and pre‑osteoblasts (1 ‑ 6). Sema5B expression may be up‑regulated in cancer cells such as human renal carcinoma clear cells, where blocking Sema5B expression attenuates viability (7). The mouse Sema5B cDNA encodes a 1093 amino acid (aa) type I transmembrane protein with a 19 aa signal sequence, a 959 aa extracellular domain (ECD), a 21 aa transmembrane sequence and a 94 aa cytoplasmic portion (8). The ECD contains one Sema domain (aa 45 ‑ 495) followed by seven consecutive TSP type 1 domains (aa 551 ‑ 952). There is a potential for at least two alternative splice forms. One shows a 32 aa substitution for aa 1041 ‑ 1093, while a second (GenBank Accession # AAH52397) contains a 29 aa insert after Val702 of the third TSP domain. Over aa 20 ‑ 776 of the SwissProt sequence that includes the Sema domain plus the first four TSP type 1 domains, mouse Sema5B shares 98% aa identity with rat, and 93 ‑ 94% aa identity with human, equine and bovine Sema5B. In the hippocampus, either full‑length or proteolytically processed and secreted Sema5B mediates neuronal synapse elimination (6). In the cortex, Sema5B appears to be inhibitory to cortical axons, but not to dorsal thalamic axons, indicating a role in corticothalamic axon guidance during development (3). Sema5B promotes the influx of extracellular calcium, which is necessary to induce neuronal growth cone collapse (9). Semaphorins typically transduce signals through transmembrane plexins. However, additional molecules such as heparin sulfate or chondroitin sulfate proteoglycans which bind TSP repeats on the class 5 family member Sema5A, can also transduce semaphorin signals (10).

References

  1. Yazdani, U. and J.R. Terman (2006) Genome Biol. 7:211.
  2. Adams, R.H. et al. (1996) Mech. Dev. 57:33.
  3. Lett, R.L.M. et al. (2009) Cerebral Cortex 19:1408.
  4. Goldberg, J.L. et al. (2004) J. Neurosci. 24:4989.
  5. Cohen, R.I. et al. (2003) J. Neurochem. 85:1262.
  6. O’Connor, T.P. et al. (2009) Neural Dev. 4:18.
  7. Hirota, E. et al. (2006) Int. J. Oncol. 29:799.
  8. SwissProt Accession # Q60519.
  9. To, K.C.W. et al. (2007) Mol. Cell. Neurosci. 36:425.
  10. Kantor, D.B. et al. (2004) Neuron 44:961.

Alternate Names

SEMA5B, SEMAG, SEMG

Entrez Gene IDs

54437 (Human); 20357 (Mouse)

Gene Symbol

SEMA5B

UniProt

AAH52397

Additional Semaphorin 5B Products

Product Documents for Recombinant Mouse Semaphorin 5B Fc Chimera Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse Semaphorin 5B Fc Chimera Protein, CF

For research use only

Related Research Areas

Proteoglycans

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