Recombinant Mouse Stanniocalcin 2/STC-2 Protein, CF

R&D Systems | Catalog # 9406-SO

R&D Systems
100% Guarantee

Key Product Details

  • R&D Systems NS0-derived Recombinant Mouse Stanniocalcin 2/STC-2 Protein (9406-SO)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

O88452

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all Stanniocalcin 2/STC-2 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Stanniocalcin 2/STC-2 protein
Thr25-Arg296, with a C-terminal 6-His tag

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Thr25

Predicted Molecular Mass

31 kDa

SDS-PAGE

33-44 kDa, reducing conditions

Activity

Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells.
The ED50 for this effect is 75-450 ng/ml in the presence of 10 ng/ml of Recombinant Mouse Wnt‑3a (Catalog # 1324-WN).

Scientific Data Images for Recombinant Mouse Stanniocalcin 2/STC-2 Protein, CF

Recombinant Mouse Stanniocalcin 2/STC-2 Protein Bioactivity

Recombinant Mouse Stanniocalcin 2/STC-2 Protein Bioactivity

Recombinant Mouse Stanniocalcin 2/STC-2 (Catalog # 9406-SO) inhibits Recombinant Mouse Wnt-3a induced alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells. The ED50 for this effect is 75-450 ng/mL in the presence of 10 ng/mL of Recombinant Mouse Wnt-3a (Catalog # 1324-WN).

Formulation, Preparation, and Storage

9406-SO
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Stanniocalcin 2/STC-2

Mouse STC-2 (Stanniocalcin-2) is a secreted disulfide-linked homodimeric glycoprotein hormone that is related to the STC protein. It is first discovered from corpuscles of stannous, an endocrine gland unique to bony fish (1-3). The 272 amino acid (aa) mature mouse STC-2 has an 86% homology with the human form. STC‑2 has been described as having a role in human adult height and is involved in the GIANT consortium (4, 5). STC-2 is expressed in a wide variety of tissues. It regulates calcium and phosphate homeostasis (1-3), enhances mesenchymal stem cell survival (6), promotes cell proliferation in cervical cancer (7), suppresses breast cancer cell migration and invasion (8), promotes osteoblast differentiation (9), and inhibits longitudinal bone growth directly at the growth plate (10). It is also a biomarker for cervical and lung cancers (11, 12).Fform

References

  1. Wagner, G.F. et al. (1995) Proc. Natl. Acad. Sci. 92:1871.
  2. Zhang, J. et al. (1998) Protein Expression and Purification. 12:390.
  3. Takei, Y. et al. (2012) Bone. 50:998.
  4. Marouli, E. et al. (2017) Nature 542:186.
  5. Holmes, D. (2017) Nature Reviews Endocrinology. 13:190.
  6. Kim PH, et al. (2015) BMB Rep. 48:702.
  7. Wang Y, et al. (2015) Biochem Biophys Res Commun. 466:362.
  8. Hou J, et al. (2015) PLoS One. 10:e0122179.
  9. Zhou J, et al. (2016) Mol Med Rep. 14:5653.
  10. Wu S, et al. (2006) J Biol Chem. 281:5120.
  11. Shen XJ, et al. (2014) Int J Clin Exp Pathol. 7: 8770.
  12. Na SS, et al. (2015) Biochim Biophys Acta. 1854:668.

Alternate Names

STC-2, STC2, STCRP

Entrez Gene IDs

8614 (Human); 20856 (Mouse)

Gene Symbol

STC2

UniProt

O88452

Additional Stanniocalcin 2/STC-2 Products

Product Documents for Recombinant Mouse Stanniocalcin 2/STC-2 Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse Stanniocalcin 2/STC-2 Protein, CF

For research use only

Customer Reviews for Recombinant Mouse Stanniocalcin 2/STC-2 Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Mouse Stanniocalcin 2/STC-2 Protein, CF and earn rewards!

Have you used Recombinant Mouse Stanniocalcin 2/STC-2 Protein, CF?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Proteins and Enzymes