Recombinant Mouse TrkB Fc Chimera Protein, CF

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Recombinant Mouse TrkB Fc Chimera Protein Bioactivity
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Product Details
Citations (2)

Recombinant Mouse TrkB Fc Chimera Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its ability to inhibit BDNF-induced proliferation of BaF‑TrkB‑BD mouse pro‑B cells transfected with TrkB. The ED50 for this effect is 0.05-0.4 μg/mL.
Mouse myeloma cell line, NS0-derived mouse TrkB protein
Mouse TrkB
Accession # P15209
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
72 kDa
90-120 kDa, under reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Mouse TrkB Fc Chimera Protein Bioactivity View Larger

Recombinant Mouse TrkB Fc Chimera (Catalog # 10266-TB) inhibits BDNF-induced proliferation of BaF3 mouse pro-B cells transfected with human TrkB. The ED50 for this effect is 0.05-0.4 µg/mL in the presence of 16 ng/mL of Recombinant Human BDNF (Catalog # 248-BDB).

SDS-PAGE Recombinant Mouse TrkB Fc Chimera Protein SDS-PAGE View Larger

2 μg/lane of Recombinant Mouse TrkB Fc Chimera (Catalog # 10266-TB) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 90-120 kDa and 180-240 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

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Background: TrkB

Tropomyocin receptor kinase B (TrkB), also named Neurotrophic tyrosine kinase receptor type 3 (NTRK3) is a member of a nerve growth factor tyrosine kinase receptor family. There are three members of the Trk family, TrkA, TrkB and TrkC, and they bind a group of structurally related, secreted proteins termed neurotrophins, which play an important role in the development and function of the nervous system. The Trk family shares a conserved structural motif consisting of two cysteine-rich domains, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains in the extracellular region, a single transmembrane domain and an intracellular tyrosine kinase domain (3). Natural splice variants of the different Trks, lacking the first cysteine-rich domain, the first and second or all three of the leucine-rich motifs, or the tyrosine kinase domain, have been described (4). Mature mouse TrkB consists of a 398 amino acid (aa) extracellular domain (ECD) which shares 89% and 97% aa identity with human and rat TrkB, respectively. Each Trk family member exhibits different ligand specificities: TrkA binds NGF and NT-3, TrkB binds BDNF, NT-3 and NT-4/5, and TrkC only binds NT-3 (1, 2). The biological activities of the neurotrophins are mediated by binding to and activating two unrelated receptor types: the p75 neurotrophin receptor (p75NTR) and the Trk family of receptors (1, 2). P75NTR is a member of the tumor necrosis factor receptor superfamily (TNFRSF) and has been designated TNFRSF16. It binds all neurotrophins with low-affinity to transduce cellular signaling pathways that synergize or antagonize those activated by the Trk receptors. TrkB is primarily expressed in the nervous system (5). However, low levels of TrkB expression have also been observed in a wide variety of tissues (pancreas, kidneys, ovary) outside the nervous system (7). Trk receptor interactions with NGF play major roles in the development of the sympathetic nervous system and are essential for neuronal survival in vivo (8). Agonists of TrkB have shown enhanced endogenous analgesia by restoring stimuli-response activity in spinal nerve damaged rats (9) and inhibition of the Trk receptors may have a range of therapeutic implications (10).

  1. Huang, E.J. and L.F. Reichardt. (2003) Annu. Rev. Biochem. 72:609.
  2. Dechant, G. (2001) Cell Tissue Res. 305:229.
  3. Schneider, R. and M. Schweiger (1991) Oncogene 6:1807
  4. Ninkina, N. et al. (1997) J. Biol. Chem. 272:13019.
  5. Soppet, D. et al. (1991) Cell 65:895.
  6. Middlemas, D.S. et al. (1991) Mol. Cell Biol. 11:143.
  7. Shelton, D. L. et al. (1995) J. Neurosci. 15:447.
  8. Fagan, A. et al. (1996) J Neurosci. 16:6208.
  9. Yan, W. et al. (2019) J Med. Chem. 62:1731.
  10. Suto, T. et al. (2019) J Pain. 20:600.
Long Name
Neurotrophic Tyrosine Kinase Receptor B
Entrez Gene IDs
4915 (Human); 18212 (Mouse); 25054 (Rat)
Alternate Names
BDNF/NT-3 growth factors receptor; EC 2.7.10; EC; GP145-TrkB; Neurotrophic tyrosine kinase receptor type 2; neurotrophic tyrosine kinase, receptor, type 2; NTRK2; TrkB tyrosine kinase; TrkB; trk-B; TRKBBDNF-tropomyosine receptor kinase B; tyrosine kinase receptor B

Citations for Recombinant Mouse TrkB Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Sciatic nerve block downregulates the BDNF pathway to alleviate the neonatal incision-induced exaggeration of incisional pain via decreasing microglial activation
    Authors: X Ding, FF Liao, L Su, X Yang, W Yang, QH Ren, JZ Zhang, HM Wang
    Brain, Behavior, and Immunity, 2022-07-16;105(0):204-224.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  2. Age-Dependency of Neurite Outgrowth in Postnatal Mouse Cochlear Spiral Ganglion Explants
    Authors: C Frick, S Fink, D Schmidbaue, F Rousset, H Eickhoff, A Tropitzsch, B Kramer, P Senn, R Glueckert, H Rask-Ander, KH Wiesmüller, H Löwenheim, M Müller
    Brain Sci, 2020-08-21;10(9):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay


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