Recombinant Rat Fc gamma RI/CD64 Protein, CF

R&D Systems | Catalog # 9558-FC

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Key Product Details

  • R&D Systems NS0-derived Recombinant Rat Fc gamma RI/CD64 Protein (9558-FC)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

NP_001094306

Applications

Bioactivity
View all Fc gamma RI/CD64 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived rat Fc gamma RI/CD64 protein
Gln16-Pro285, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Thr21 observed & Gln16 inferred from enzymatic pyroglutamate treatment revealing Val17

Predicted Molecular Mass

31 kDa

SDS-PAGE

36-48 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Rat Fc gamma  RI/CD64 is immobilized at 1 μg/mL, 100 μL/well, it binds Biotinylated Rat IgG. The concentration of Biotinylated Rat IgG that produces 50% of the optimal binding response is 0.075-0.35 μg/mL.

Scientific Data Images for Recombinant Rat Fc gamma RI/CD64 Protein, CF

Recombinant Rat Fc gamma RI/CD64 Protein Binding Activity

Recombinant Rat Fc gamma RI/CD64 Protein Binding Activity

When Recombinant Rat Fc gamma RI/CD64 (Catalog # 9558‑FC) is immobilized at 1 μg/mL, 100 μL/well, Biotinylated Rat IgG binds with an ED50 of 0.075-0.35 μg/mL.

Formulation, Preparation, and Storage

9558-FC
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and Trehalose.
Reconstitution

Reconstitute at 1 mg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Fc gamma RI/CD64

Receptors for the Fc region of IgG (Fc gamma  Rs) are members of the Ig superfamily that function in the activation or inhibition of immune responses such as degranulation, phagocytosis, cytokine release, and B cell proliferation. There are three classes of Fc gamma  Rs, based on close relationships in their extracellular domains: Fc gamma  RI/CD64, Fc gamma RII/CD32, and Fc gamma  RIII/CD16. Each group may be encoded by multiple genes and exists in different isoforms depending on species and cell type (1-3). The CD64 proteins are high affinity receptors (~10-8-10-9 M) that are capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10-6-10-7 M) (4, 5). The high affinity of IgG by Fc gamma  RI permits the triggering of effector responses at low IgG concentrations typical of early immune response (6). The mature protein from this family consists of an extracellular domain (ECD), a transmembrane segment, and a cytoplasmic domain. Within the ECD, rat Fcɣ RI shares 78% and 64% amino acid sequence identity with mouse and human Fc gamma RI, respectively. Fc gamma RI is expressed constitutively on monocytes and macrophages and can be induced on neutrophils and eosinophils. Its expression is up-regulated during bacterial infections and sepsis (1, 5).

References

  1. Van de Winkel, J. and P. Capes (1993) Immunol. Today. 14:215.
  2. Raghaven, M. and P. Bjorkman (1996) Annu. Rev. Cell Dev. Biol. 12:181.
  3. Ravetch, J. and S. Bolland (2001) Annu. Rev. Immunol. 19:275.
  4. Chenoweth, A.M et al. (2015) Immunol. Rev. 268:175.
  5. Takai, T. (2002) Nature Rev. Immunol. 2:580.
  6. Raghaven, M. and P. Bjorkman (1996) Annu. Rev. Cell Dev. Biol. 12:181.

Long Name

Fc gamma Receptor I

Alternate Names

CD64, CD64a, FCGR1, FcgRI, FcgRIA, FCRI, FcRIA

Entrez Gene IDs

2209 (Human); 14129 (Mouse); 295279 (Rat); 102147198 (Cynomolgus Monkey)

Gene Symbol

FCGR1A

UniProt

NP_001094306

Additional Fc gamma RI/CD64 Products

Product Documents for Recombinant Rat Fc gamma RI/CD64 Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Rat Fc gamma RI/CD64 Protein, CF

For research use only

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