Recombinant Rat Fc gamma RIIB/CD32b Protein, CF

Catalog # Availability Size / Price Qty
9666-CD-100
Recombinant Rat Fc gamma RIIB/CD32b Protein, CF Data
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Recombinant Rat Fc gamma RIIB/CD32b Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Rat Fc gamma  RIIB/CD32b is immobilized on an anti-His Tag Antibody coated plate, it binds Biotinylated Rat IgG. The concentration of Biotinylated Rat IgG that produces 50% of the optimal binding response is 0.5-3 μg/mL.
Source
Mouse myeloma cell line, NS0-derived rat Fc gamma RIIB/CD32b protein
His32-Pro212, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
His32 & Gly34
Predicted Molecular Mass
21 kDa
SDS-PAGE
33-45 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9666-CD

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity View Larger

When Recombinant Rat Fc gamma RIIB/CD32b (Catalog # 9666-CD) is immobilized on an anti-His Tag Antibody coated plate, it binds Biotinylated Rat IgG with an ED50of 0.5-3 μg/mL.

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Background: Fc gamma RIIB/CD32b

Receptors for the Fc region of IgG (Fc gamma Rs) are members of the Ig superfamily that function in the activation or inhibition of immune responses such as degranulation, phagocytosis, ADCC (antibody-dependent cellular toxicity), cytokine release, and B cell proliferation (1). The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma RI/CD64, Fc gamma RII/CD32, and Fc gamma RIII/CD16. Fc gamma RII A and C contain activating cytoplasmic ITAM sequences, while Fc gamma RIIB contains an inhibitory cytoplasmic ITIM sequence. The Fc gamma RI proteins are high affinity receptors (~10-8-10-9 M) capable of binding monomeric IgG, whereas the Fc gamma RII and RIII proteins bind IgG with lower affinities (~10-6-10-7 M) and only recognize IgG aggregates surrounding multivalent antigens (1). The mature rat Fc gamma RIIB consists of a 181 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 52 aa cytoplasmic domain. Within the ECD, rat Fc gamma RIIB shares 58% and 78 % aa sequence identity with human and mouse Fc gamma RIIB, respectively. It binds to both cynomolgus and human IgG subclasses 1-4 (2). Fc gamma RIIB is expressed on B cells, monocytes, dendritic cells, neutrophils, mast cells, and basophils (1). Ligation of Fc gamma RIIB triggers signaling that suppresses B cell expansion, plasma cell differentiation, production of autoimmune rheumatoid factors, and down-regulation of TLR4 with reduced LPS responsiveness (3-6). The coordinated functioning of activating and inhibitory receptors is necessary for successful initiation, amplification, and termination of immune responses (7).

References
  1. Nimmerjahn, F. and J.V. Ravetch (2008) Nat. Rev. Immunol. 8:34.
  2. Warncke, M. et al. (2012) J. Immunol. 188:4405.
  3. Takai, T. et al. (1996) Nature 379:346.
  4. Karnell, J.L. et al. (2014) J. Immunol. 192:1480.
  5. Moll, T. et al. (2004) J. Immunol. 173:4724.
  6. Zhang, Y. et al. (2009) J. Immunol. 182:554.
  7. Ravetch, J. and L. Lanier (2000) Science 290:84.
Long Name
Fc gamma Receptor II
Entrez Gene IDs
2213 (Human); 14130 (Mouse); 289211 (Rat); 102139530 (Cynomolgus Monkey)
Alternate Names
CD32 antigen; CD32b; CD32Fc fragment of IgG, low affinity II, receptor for (CD32); CDw32; Fc fragment of IgG, low affinity IIb, receptor (CD32); Fc fragment of IgG, low affinity IIb, receptor for (CD32); Fc gamma RIIB; FCG2; Fc-gamma RII-b; fc-gamma-RIIb; FCGR2; FCGR2B; FcgRIIB; FCRIIB; fcRII-b; IGFR2; IgG Fc receptor II-b; low affinity immunoglobulin gamma Fc region receptor II-b

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