Recombinant Viral wnvNS1 His-tag Protein, CF

Catalog #: 10945-SE Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10945-SE-100

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Recombinant Viral wnvNS1 His-tag Protein SDS-PAGE.

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Viral wnvNS1 His-tag Protein, CF Summary

R&D Systems HEK293-derived Recombinant Viral wnvNS1 His-tag Protein (10945-SE)
Quality control testing to verify active proteins with lot specific assays by in-house scientists
All R&D Systems proteins are covered with a 100% guarantee

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Bioassay data are not available.

Source

Human embryonic kidney cell, HEK293-derived viral wnvNS1 Protein protein
Asp1-Ala352, with a C-terminal 6-His tag

Accession #

NP_776015.1

N-terminal Sequence
Analysis

Asp1

Predicted Molecular Mass

40 kDa

SDS-PAGE

52-62 kDa, under reducing conditions.

Product Datasheets

10945-SE

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10945-SE

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

SDS-PAGE

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2 μg/lane of Recombinant Viral wnvNS1 His-tag Protein (Catalog # 10945-SE) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 52-62 kDa.

Reconstitution Calculator

Background: wnvNS1 Protein

The West Nile Virus [WNV] is a neurotropic human pathogen. This virus belongs to the flavivirus genus which is in the Flaviviridae family along with the Dengue virus (DENV), Yellow fever virus (YFV), Japanese encephalitis virus (JEV), and Zika virus. The West Nile Virus is a mosquito-borne virus and affects many other animals besides humans, such as birds, reptiles and other mammals (1). The West Nile Virus is an enveloped virion that contains a positive single stranded RNA. The viral RNA is translated to produce 3 structural and 7 nonstructural proteins. The structural proteins are capsid (C), envelope (E), and precursor membrane proteins (prM) and the nonstructural proteins include NS1, NS2A, NS2B, NS3, NS4A, NS4B, and NS5. The structural proteins are encoded at the 5' end of the RNA strand and serve the function of virus entry and encapsulation of the genome (2). WNV NS1 protein is a glycoprotein ~50 kDa. It shares 55.7% and 54.7% homology with NS1 from DENV2 and Zika virus. NS1 exists as a monomer, dimer, and hexamer, depending on its localization (3-5). Glycosylation is critical for its functions (6). Although no known role in viron assembly, NS1 is important for virulence and viral replication (7). Intracellular NS1 is reported to interact with various host proteins, such as 60S ribosome subunit, hnRNP C1/C2 and hnRNP K to assist virus replication (8-10). TLR3 receptor is reported to mediate WNV entry into the brain (11). Secreted and cell surface associated NS1 is highly immunogenic, making it an important biomarker and vaccine target (12-14).

References