Rhobo6 ECM Dye

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8876/76U

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Rhobo6 ECM Dye | Live-cell Imaging
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Description: Fluorogenic probe for labeling the extracellular matrix (ECM) in live tissues

Chemical Name: (E)-2-(6-((2-Boronobenzyl)amino)-3-((2-boronobenzyl)iminio)-3H-xanthen-9-yl)-4-carboxybenzoate trifluoroacetate

Purity: ≥90%

Product Details
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Biological Activity

Key information: Rhobo6 ECM Dye is a cell-impermeable fluorescent probe for labeling the extracellular matrix (ECM) in live tissues. It contains a phenylboronic acid group that binds reversibly to diols commonly found in ECM glycans resulting in a significant increase in fluorescence and a red shift in emission spectra.

Used for: labeling the ECM in live tissues.

Application: fluorescent microscopy including 2P and STED microscopy. Enables wash-free visualization of ECM in vitro and in vivo and is suitable for long-term imaging studies.

Properties and Photophysical Data: Rhobo6 provides broad substrate profile, superior tissue penetration, and negligible photobleaching. Excitation and emission maxima (λ) are 535 nm and 561 nm, respectively; quantum yield = 0.75; extinction coefficient = 97,500 M-1cm-1.

Please refer to this guide for further information on how to use this product.

Optical Data for Rhobo6 ECM Dye

Rhobo6 ECM Dye Dye Spectra
λabs 522 nm
λem 547 nm
Extinction Coefficient (ε) 66,400 M-1cm-1
Quantum Yield (φ) 0.61
Cell Permeable No

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Technical Data

M.Wt:
756.26
Formula:
C35H28B2N2O9.CF3CO2H
Solubility:
Soluble to 10 mM in DMSO
Purity:
≥90%
Storage:
Store at -20°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Additional Information

Licensing Caveats:
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

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