Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI9H2
View all RhoGAP Primary Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 948-1261 of human ARHGAP5 (NP_001164) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for RhoGAP Antibody (OTI9H2)

RhoGAP Antibody (OTI9H2)

Western Blot:RhoGAP Antibody (OTI9H2)[NBP3-44958]

Western Blot:RhoGAP Antibody (OTI9H2)[NBP3-44958]
RhoGAP Antibody (OTI9H2)

Western Blot:RhoGAP Antibody (OTI9H2)[NBP3-44958]

Western Blot:RhoGAP Antibody (OTI9H2)[NBP3-44958]
RhoGAP Antibody (OTI9H2) Western Blot;RhoGAP Antibody (OTI9H2)[NBP3-44958]-

Western Blot;RhoGAP Antibody (OTI9H2)[NBP3-44958]-

HEK293T cells were transfected with the pCMV6-ENTRY control ( Left lane) or pCMV6-ENTRY ARHGAP5 ( Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARHGAP5 (1:2000).
RhoGAP Antibody (OTI9H2) Western Blot;RhoGAP Antibody (OTI9H2)[NBP3-44958]-

Western Blot;RhoGAP Antibody (OTI9H2)[NBP3-44958]-

Western blot analysis of extracts (35ug) from 8 different cell lines by using anti-ARHGAP5 monoclonal antibody (1:500).

Applications for RhoGAP Antibody (OTI9H2)

Application
Recommended Usage

Western Blot

1:500-2000

Formulation, Preparation, and Storage

Purification

>95%

Formulation

PBS (pH 7.3) 1% BSA, 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 degrees C. Avoid freeze/thaw cycles.

Background: RhoGAP

The p190 Rho GTPase activating protein RhoGAP) family includes two closely related proteins, p190-A and p190-B. p190-B contains an N-terminal GTPase domain and a C-terminal Rho-GAP domain (1). p190-B can be directly phosphorylated on a single identified tyrosine residue by the activated insulin and IGF-1 receptors. This phosphorylation causes p190-B to translocate to the lipid rafts of the plasma membrane, where it can facilitate the inactivation of the Rho GTPase. P190-B also has been shown to be tyrosine phosphorylated by c-Src and v-Src. Additionall; the GAP domain of p190-B as shown to attenuate signal-transducing activity of Rac, Rho and CDC42 (2-3).

Alternate Names

growth factor independent 2, p100 RasGAP-associated p105 protein, p105 RhoGAP, p190-BGFI2, p190BRhoGAP, Rho GTPase activating protein 5, rho GTPase-activating protein 5, RHOGAP5, Rho-type GTPase-activating protein 5

Gene Symbol

ARHGAP5

Additional RhoGAP Products

Product Documents for RhoGAP Antibody (OTI9H2)

Certificate of Analysis

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Product Specific Notices for RhoGAP Antibody (OTI9H2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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