Ribosomal Protein S6/RPS6 Antibody (A6B8-R)

Novus Biologicals | Catalog # NBP3-32919

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 Clone # A6B8-R expressed in HEK293
View all Ribosomal Protein S6/RPS6 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein within human Ribosomal Protein S6/RPS6 aa 1-150. (Uniprot: P62753)

Localization

Cytosol, Endoplasmic reticulum, Nucleus.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Ribosomal Protein S6/RPS6 Antibody (A6B8-R)

Ribosomal Protein S6/RPS6 Antibody (A6B8-R) Western Blot: Ribosomal Protein S6/RPS6 Antibody (A6B8-R) [NBP3-32919]

Western Blot: Ribosomal Protein S6/RPS6 Antibody (A6B8-R) [NBP3-32919]

Western blot analysis on different lysates with NBP3-32919 at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: HepG2 cell lysate Lane 4: HEK-293 cell lysate Lane 5: HCT 116 cell lysate Lane 6: THP-1 cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 29 kDa Observed band size: 29 kDa Exposure time: 1 minute 22 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32919 at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat anti-Mouse IgG [HRP] at 1/50,000 dilution was used for 1 hour at room temperature.

Applications for Ribosomal Protein S6/RPS6 Antibody (A6B8-R)

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH7.4), 0.1% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Ribosomal Protein S6/RPS6

RPS6, also known as Ribosomal Protein S6, is a 29kDa 249 amino acid protein. The primary function of RPS6 is to control cell growth during the selective translation of mRNA. Current research is being conducted on RPS6 due to its relationship tomyeloid leukemia, Kaposi's sarcoma, cutaneous t cell lymphoma, lymphangioleiomyomatosis, congestive heart failure and b-cell lymphomas. RPS6 has been linked to the pathogenesis, transport, cell growth and cell death pathways where it interacts with RPS2, RPS3, RPL11, RPL26 and RPL35.

Alternate Names

RPS6

Gene Symbol

RPS6

Additional Ribosomal Protein S6/RPS6 Products

Product Documents for Ribosomal Protein S6/RPS6 Antibody (A6B8-R)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Ribosomal Protein S6/RPS6 Antibody (A6B8-R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Interferons
Translation Regulation

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